摘要
通过MTT法和空斑实验法评价了17种食药用菌提取物对单纯疱疹病毒1型(herpes simplex virus 1,HSV-1)和肠道病毒71型(enterovirus 71,EV71)的两个不同来源毒株EV71-CSFI4、EV71-PTPS的抑制效果。结果表明:皱盖假芝(Amauroder marudis)、枣翘鳞肉齿菌(Sarcodon imbricatus)水提物对3种病毒均具有较好的抑制效果,皱盖假芝水提物对HSV-1、 EV71-CSFI4、EV71-PTPS的治疗指数分别为32.17、30.05、21.55;枣翘鳞肉齿菌水提物对EV71-CSFI4、EV71-PTPS的治疗指数较高,分别为95.35、112.22。研究结果提示皱盖假芝、枣翘鳞肉齿菌水提物具有进一步开发抗病毒产品的潜力。
Using cytotoxicity assay(MTT)and plaque reduction assay,extracts of 17 kinds of edible fungi were evaluated for their antiviral efficacy against the herpes simplex virus 1(HSV-1)and 2 strains of enterovirus-71(Ev71),EV71-CSFI4 and EV71-PTPS in vitro.The results showed that water extracts of Amauroder marudis and Sarcodon imbricatus exhibited good antiviral activity.The therapeutic indices(TIs)of A.marudis water extract against HSV-1,EV71-CSFI4 and EV71-PTPS were 32.17,30.05 and 21.55,respectively.The therapeutic indices(TIs)of S.imbricatus water extract against EV71-CSFI4 and EV71-PTPS were 95.35 and 112.22,respectively.Therefore,A.marudis and S.imbricatus showed potential antiviral ability to be developed into antiviral therapeutics.
作者
蔡超
雷苏炜
陈少丹
谢意珍
潘鸿辉
王弋
CAI Chao;LEI Suwei;CHEN Shaodan;XIE Yizhen;PAN Honghui;WANG Yi(Guangdong Institute of Microbiology Guangdong Academy of Sciences,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangzhou,Guangdong 510000,China;Yuewei Edible Fungi Technology Co.Ltd.,Guangzhou,Guangdong 510070,China;The First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou,Guangdong 510062;Guangzhou Yuanbo Biotechnology Limited Company,Guangzhou,Guangdong 510663,China)
出处
《食用菌学报》
CSCD
北大核心
2020年第1期75-84,共10页
Acta Edulis Fungi
基金
广东省自然科学基金(2018A030313887)
广东省科学院引进创新创业团队项目(2016GDASRC-0102)
广东省农业科技创新及推广项目(2019KJ103)
广东省科技计划项目(2014A080802007)。
关键词
食药用菌
提取物
单纯疱疹病毒
肠道病毒71型
抗病毒
治疗指数
Edible and medicinal fungi
extract
herpes simplex virus
enterovirus 71
antiviral
therapeutic index
