摘要
为了检测不同提取方法获得的浒苔提取物浒苔多糖1、浒苔多糖2、浒苔多糖3和浒苔多糖硒的体外抗禽流感病毒活性,试验将4种浒苔多糖用细胞维持液倍比稀释,以金刚烷胺、病毒灵、黄芪多糖和紫锥菊提取物为对照,以犬肾细胞(MDCK)为细胞模型,观察细胞病变效应(CPE),并采用MTT法研究4种浒苔多糖对H9N2禽流感病毒的直接杀灭、抑制病毒复制和阻断病毒吸附的治疗指数。结果表明:浒苔多糖1和浒苔多糖2直接杀灭H9N2禽流感病毒作用治疗指数分别为7.33和7.29,该指数低于金刚烷胺(33.56)、病毒灵(36.00)和黄芪多糖(15.78),高于紫锥菊提取物(6.92);浒苔多糖1和浒苔多糖2抑制H9N2禽流感病毒复制作用的治疗指数分别为5.78和6.13,该指数低于金刚烷胺(22.68)、病毒灵(26.71)和黄芪多糖(10.18),高于紫锥菊提取物(5.41);浒苔多糖1和浒苔多糖2阻断H9N2禽流感病毒吸附作用的治疗指数分别为13.83和12.75,该指数低于金刚烷胺(46.61)、病毒灵(55.20)和黄芪多糖(16.25),高于紫锥菊提取物(11.22);浒苔多糖3和浒苔多糖硒不具有直接杀灭病毒、抑制病毒复制和阻断病毒吸附的作用。说明浒苔多糖1和浒苔多糖2具有体外抗禽流感病毒活性,表现为对H9N2禽流感病毒具有直接杀灭、抑制病毒复制和阻断吸附的作用,浒苔多糖3和浒苔多糖硒无体外抗禽流感病毒活性。
In order to detect the anti-avian influenza virus activity of Brassica polysaccharide 1,Brassica polysaccharide 2,Brassica polysaccharide 3 and Brassica polysaccharide selenium obtained by different extraction methods in vitro.The four kinds of canola polysaccharides were diluted with cell maintenance solution,and amantadine,virion,astragalus polysaccharide and echinacea extract were used as controls,and the canine kidney cells(MDCK)were used as the cell model to observe the cytopathic effect(CPE).The MTT method was used to detect the therapeutic index of four kinds of Brassica polysaccharides for direct killing of H9 N2 avian influenza virus,inhibition of viral replication and blocking of virus adsorption.The results showed that the therapeutic index of direct application of Brassica polysaccharide 1 and Brassica polysaccharide 2 directly killing H9 N2 avian influenza virus was 7.33 and 7.29,respectively,which was lower than that of amantadine(33.56),virgin(36.00)and astragalus polysaccharide(15.78),higher than Echinacea extract(6.92);The therapeutic indices of the inhibition of H9 N2 avian influenza virus replication by Brassica polysaccharide 1 and Brassica polysaccharide 2 were 5.78 and 6.13,respectively,which was lower than that of amantadine(22.68),virgin(26.71)and astragalus polysaccharides(10.18),higher than Echinacea extract(5.41).The therapeutic indices of the adsorption of H9 N2 avian influenza virus by Brassica polysaccharide 1 and Brassica polysaccharide 2 were 13.83 and 12.75,respectively,which was lower than that of amantadine(46.61),virgin(55.20)and astragalus polysaccharide(16.25),higher than Echinacea extract(11.22);The therapeutic indices of direct killing H9 N2 avian influenza virus,inhibition of viral replication and blocking of virus adsorption of Brassica polysaccharide 3 and Brassica polysaccharide selenium were zero.It indicated that Brassica polysaccharide 1 and Brassica polysaccharide 2 had anti-avian influenza virus activity in vitro,which had the effect of directly killing,inhibiting virus replication and blocking adsorption of H9 N2 avian influenza virus.Brassica polysaccharide 3 and Brassica Polysaccharide selenium had no anti-avian influenza virus activity in vitro.
作者
于超
杜恒裔
谭子超
刘晓龙
王述柏
YU Chao;DU Hengyi;TAN Zichao;LIU Xiaolong;WANG Shubai(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2020年第1期106-109,113,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家海洋局“十三五”海洋经济创新发展示范工作产业链协同创新类项目“年产5000吨海藻农用生物制品关键技术集成及产业示范及山东省现代农业产业技术体系家禽产业创新团队建设项目”(SDAIT-11-14)。
关键词
浒苔多糖
浒苔多糖硒
禽流感病毒
MDCK
体外抗病毒活性
治疗指数
Brassica polysaccharide
Brassica polysaccharide selenium
avian influenza virus
MDCK
antiviral activity in vitro
therapeutic index
