摘要
目的了解临床感染分离的金黄色葡萄球菌的耐药性,为其感染诊治选择合理抗菌药物提供理论参考。方法将2015年3月~2018年5月本院送检临床感染标本,按照《全国临床检验操作规程》标准进行金黄色葡萄球菌分离,接种后于36℃、5%CO2环境培养、纯化、鉴定,K-B法判别药敏结果,并采用PCR检测耐药基因。结果840份临床感染标本共分离出金黄色葡萄球菌168株,金黄色葡萄球菌对青霉素G、阿莫西林、氯霉素、头孢噻肟、红霉素、四环素、苯唑西林、庆大霉素、环丙沙星、克林霉素、诺氟沙星、左氧氟沙星、头孢吡肟、万古霉素的耐药率分别为89.29%、83.33%、69.05%、61.90%、53.57%、41.67%、33.33%、28.57%、26.19%、23.81%、22.62%、14.29%、2.38%和0,中介率分别为4.76%、5.95%、9.52%、10.71%、15.48%、20.24%、13.10%、53.57%、42.86%、21.43%、19.05%、11.90%、11.90%和0,敏感率分别为5.95%、10.71%、21.43%、27.38%、30.95%、38.10%、53.57%、17.86%、30.96%、54.76%、58.33%、73.81%、85.71%和100.00%,在抗感染治疗中可优先考虑使用万古霉素等敏感药物。结论临床感染金黄色葡萄球菌对常用抗生素有耐药性,应根据药敏试验结果正确选用有效抗菌药物进行治疗,对减少细菌耐药性的发生有重要意义。
Objective To understand the drug resistance of staphylococcus aureus isolated from clinical infection and provide theoretical reference for reasonable use of antibiotics in diagnosis and treatment of infection.Methods Staphylococcus aureus were isolated according to national clinical laboratory procedures from Clinical infection specimens submitted by our hospital from March 2015 to May 2018.After inoculation,culture,purification and identification at 36℃and 5%CO2 were performed.The K-B method was applied to distinguish sensitivity,and PCR was used to detect resistant genes.Results Among 168 strains of staphylococcus aureus isolated from 840 clinical infection specimens,resistance rates of penicillin G,amoxicillin,chloramphenicol,cefotaxime,erythromycin,tetracycline,oxacillin,gentamicin,erythromycin,Ciprofloxacin,norfloxacin,levofloxacin,cefepime,vancomycin were 89.29%,83.33%,69.05%,61.90%,53.57%,41.67%,33.33%,28.57%,26.19%,23.81%,22.62%,14.29%,2.38%,and 0 respectively;intermediate susceptibility rates were 4.76%,5.95%,9.52%,10.71%,15.48%,20.24%,13.10%,53.57%,42.86%,21.43%,19.05%,11.90%,11.90%,and 0 respectively.Sensitivity rates were 5.95%,10.71%,21.43%,27.38%,30.95%,38.10%,53.57%,17.86%,30.96%,54.76%,58.33%,73.81%,85.71%,and 100.00%respectively.Priority should be given to sensitive drugs such as vancomycin in anti-infection treatment.Conclusion Staphylococcus aureus is resistant to commonly used antibiotics in clinical infection.Selecting effective antibiotics according to the result of drug sensitive test is of great significance to reduce the occurrence of bacterial resistance.
作者
杨波
张灿灿
YANG Bo;ZHANG Cancan(Department of Laboratory,Xinjiang Production and Construction Corps Fourth Division Hospital,Yining 835000,China)
出处
《中国现代医生》
2020年第2期139-142,共4页
China Modern Doctor
关键词
金黄色葡萄球菌
耐药基因
耐药性
K-B法
Staphylococcus aureus
Resistant genes
Drug resistance
K-B method
