摘要
目的:研究PM2.5和AngⅡ的共同干预下,人脐静脉血管内皮细胞的凋亡率及可能的凋亡机制。方法:体外培养人脐静脉内皮细胞株,分别以不同浓度PM2.5、AngⅡ、PM2.5+AngⅡ处理24、48 h,CCK-8法检测细胞活性,流式细胞术检测细胞凋亡率,Western blot法检测凋亡相关蛋白p-AKT的表达变化。结果:48 h CCK-8结果显示,PM2.5在100.00、200.00、800.00 μg/mL时、AngⅡ在20.00 μmol/L时与对应组别的PM2.5+AngⅡ组比较,差异均有统计学意义(P<0.05)。进一步的流式结果显示,48 h时PM2.5+AngⅡ组凋亡率与单PM2.5或AngⅡ处理比较,差异均有统计学意义(P<0.01,P<0.001)。Western Blot结果显示,PM2.5+AngⅡ处理比单PM2.5或单AngⅡ处理后P-AKT的表达量下调,差异均有统计学意义(P<0.005)。结论:本研究表明体外实验中,PM2.5对AngⅡ诱导血管内皮细胞凋亡具有促进作用,并可能通过PI3K/Akt/eNOS信号通路来实现。
Objective:To study the apoptosis rate and possible apoptosis mechanism of Human Umbilical Vein Endothelial Cells (HUVEC) under the co-intervention of PM2.5 and AngⅡ.Method:HUVEC were treated with different concentrations of PM2.5,AngⅡand PM2.5 + AngⅡ for 24 and 48 h.The cell activity was detected by CCK-8 assay and the apoptosis rate was detected by flow cytometry (FCM).The expression of apoptosis-related protein p-AKT was detected by Western blot.Result:The results of CCK-8 showed that there were significant differences between PM2.5 at 100.00,200.00,800.00 μg/mL and AngⅡ at 20.00 μmol/L and PM2.5 + AngⅡ (P<0.05).The further results of FCM showed that there were differences significantly between PM2.5 with AngⅡ and single PM2.5 or single AngⅡ treatment group in the apoptosis rate (P<0.01,P<0.001).The results of Western Blot showed that the expression of p-AKT in PM2.5 with AngⅡ treatment group was significantly lower than that in single PM2.5 or single AngⅡ treatment group (P<0.005).Conclusion:This study shows that in vitro experiments,PM2.5 can promote the apoptosis effect of AngⅡ to HUVEC.It may be achieved through PI3K/Akt/eNOS signaling pathway.
作者
李岗
邓宇亭
陈冬梅
LI Gang;DENG Yuting;CHEN Dongmei(School of Basic Medicine,Shenyang Medical College,Shenyang 110034,China)
出处
《中国医学创新》
CAS
2020年第4期1-6,共6页
Medical Innovation of China
基金
沈阳医学院重点实验室研究基金(20165051)。
