摘要
目的监测绵阳市外环境中禽类流行性感冒(禽流感)病毒的分布状况,为人禽流感防控提供参考依据。方法本中心采用real-time RT-PCR方法对外环境标本进行流感病毒A型核酸检测,再将阳性标本进行H5、H7和H9分型,采用χ^2检验比较定性资料。结果在816份样本中,甲型流感病毒阳性标本88份,检出率为10.78%;分型实验中,H5型、H7型、H9/H5型+H9型分别检出2份、0份、76份和2份,其阳性检出率依次为0.24%、0、9.31%、0.24%,未分型成功8份,占总样本的0.98%。结论绵阳市12个县市区外环境均存在禽流感病毒污染,有人感染禽流感风险;需加强对外环境及高危人群禽流感监测,并规范对市场的管理机制,落实消毒和检疫准入等预防措施。
Objective To monitor the distribution of avian influenza virus in the external environment of Mianyang City,and provide reference for the prevention and control of human avian influenza.Methods Real-time fluorescent quantitative PCR is adopted in our center to detect influenza A virus in the external environment.The positive samples were classified into H5,H7 and H9,qualitative data were compared usingχ^2 test.Results Among the 816 samples,88 were positive for influenza A virus,and the detection rate was 10.78%.In the typing experiment,H5,H7,H9 and H5+H9 were respectively detected in 2 cases,0 case,76 cases and 2 cases.The positive detection rate was 0.24%,0,9.31%and 0.24%,and 8 cases were untyped,accounting for 0.98%of the total sample.Conclusion There is avian influenza virus contamination in the external environment of Mianyang,and some people are infected with avian influenza.It is necessary to strengthen the monitoring of avian influenza in the external environment and high-risk population,and regulate the market management mechanism and implement preventive measures such as disinfection and quarantine access.
作者
文艳
李永清
陈果
杨小蓉
李仕一
WEN Yan;LI Yong-qing;CHEN Guo;YANG Xiao-rong;LI Shi-yi(Mianyang Center for Disease Control and Prevention,Mianyang,Sichuan 621000,China)
出处
《中国卫生检验杂志》
CAS
2020年第6期761-763,766,共4页
Chinese Journal of Health Laboratory Technology
关键词
禽流感
监测
外环境
Avian influenza
Monitoring
External environment
