摘要
为了探明小麦‘TcLr19’抵抗小麦叶锈菌侵染的内在分子机制,挖掘与抗叶锈菌密切相关的功能基因,对接种叶锈菌后的小麦材料‘TcLr19’进行Illumina NextSeq500转录组测序。筛选到差异表达的基因(Differential expressiongenes,DEGs)8970个,其中上调差异表达基因4953个,下调差异表达基因4017个。差异基因通过GO(Gene Ontology)功能分类和KEGG(Kyoto Encyclopedia of Gene and Genomes)Pathway富集分析,被归于1 790个GO类别,定位到84个代谢通路中。采用实时荧光定量PCR(Quantitative Real-time PCR,qRT-PCR)对转录组分析结果进行验证,结果表明,qRT-PCR与RNA-seq分析结果基本一致,为小麦TcLr19抗小麦叶锈菌新基因的挖掘提供了可靠的参考资料。
To understand the internal molecular mechanism of wheat TcLr19 under the stress of leaf rust pathogen,and identify genes closely related to resistance to leaf rust pathogen,Illumina NextSeq500,a transcriptome sequencing technology was applied to TcLr19 inoculated by Puccinia triticina.8970 Differential Expression Genes(DEGs)were obtained,of which 4,953 were up-regulated and 4017 were down-regulated.These DEGs were classified into 1790 GO categories and 84 different metabolic pathways using GO classification and KEGG Pathway cluster analysis,respectively.Quantitative Real-time PCR(qRT-PCR)was used to verify the results,and the results of qRT-PCR was similar to that of the RNA-seq.The analysis results above provide a reliable reference date for the discovery of new genes of wheat TcLr19 resistant to wheat leaf rust pathogen.
作者
张艳俊
杨毅清
袁心悦
王海燕
刘大群
ZHANG Yanjun;YANG Yiqing;YUAN Xinyue;WANG Haiyan;LIU Daqun(College of Plant Protection,Hebei Agricultural University/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding 071001,China;Graduate School,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2020年第1期11-16,共6页
Journal of Hebei Agricultural University
基金
国家自然科学基金(31501623)
河北省高等学校科学技术研究项目(QN2015171)。
