摘要
为了解云南省猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)和猪伪狂犬病病毒(Pseudorabies virus,PRV)的流行情况,试验采集云南省不同地区具有呼吸道症状的猪血清样品174份,首先建立PRRSV-GP基因和PRV-gB基因的双重PCR检测方法,然后运用建立的双重PCR方法和ELISA抗体检测试剂盒对PRRSV-GP和PRV-gB的阳性率及抗体水平进行检测分析。结果表明:试验建立了最低检测限度分别为1.48 ng/μL和0.67 ng/μL的PRRSV-GP和PRV-gB的双重PCR检测方法;单一PCR和双重PCR检测的PRRSV-GP阳性率为45.4%(79/174),PRV-gB阳性率为75.86%(132/174),混合检出率为5.17%(9/179);ELISA检测试剂盒检测的gE阳性率为0(0/174),PRRSV和PRV-gB的阳性率结果与单一PCR、双重PCR检测结果一致。说明试验构建的双重PCR方法有良好的敏感性和特异性;云南省部分地区PRRSV抗体阳性率低且免疫效果较差,PRV抗体阳性率相对较高且免疫效果良好。
In order to understand the prevalence of Porcine Reproductive and Respiratory Syndrome virus(PRRSV)and Pseudorabies virus(PRV)in Yunnan province,serum samples from 174 pigs with respiratory symptoms in different areas of Yunnan province were collected.First ly,a dual PCR assay for the PRRSV-GP gene and the PRV-gB gene was established,and then the positive rate and antibody level of PRRSV-GP and PRV-gB were detected and analyzed by using the established dual PCR method and ELISA antibody detection kit.The results showed that the PRRSV-GP and PRV-gB double PCR assays with the lowest detection limits of 1.48 ng/μL and 0.67 ng/μL were established.The single-PCR and double-PCR test results indicated that the positive rate of PRRSV-GP was 45.4%(79/174),PRV-gB positive rate was 75.86%(132/174),mixed detection rate was 5.17%(9/179);ELISA test kit detected gE positive rate was 0(0/174).The positive rate results of PRRSV and PRV-gB were consistent with the results of single-PCR and double-PCR.It indicated that the double PCR method constructed by the experiment had good sensitivity and specificity.The positive rate of PRRSV antibody was low in some areas of Yunnan province,and the positive rate of PRV antibody was relatively high and the immune effect was good.
作者
王浩
单春兰
王琛
高洪
严玉霖
赵汝
敖平星
高利波
刘超英
富国文
高斌
景麟稀
茶金龙
杨伟
WANG Hao;SHAN Chunlan;WANG Chen;GAO Hong;YAN Yulin;ZHAO Ru;AO Pingxing;GAO Libo;LIU Chaoying;FU Guowen;GAO Bin;JING Linxi;CHA Jinlong;YANG Wei(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2020年第3期75-79,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31660704
31260594)
云南省现代农业生猪产业技术体系建设项目(云财农〔2009〕171号)
云南省高校科技创新团队支持计划项目(云教科〔2011〕14号)。
