摘要
植物液泡铁离子转运蛋白可维持细胞内铁离子含量,保证细胞正常生长,在植株抵抗盐碱环境过程中发挥重要作用。在大豆中克隆得到液泡铁离子转运蛋白同源基因GmVIT1,蛋白结构域分析结果表明,GmVIT1属于CCC1蛋白超家族,具有高度保守的结构域。研究发现GmVIT1具有跨膜结构,并利用YFP荧光蛋白确定GmVIT1蛋白定位在液泡膜上;实时荧光定量PCR(qRT-PCR)显示,GmVIT1在大豆根、茎、叶、花、荚和种子中均表达,花中mRNA丰度相对较高;胁迫后发现,GmVIT1的mRNA积累受ABA负调控,盐胁迫下基因显著上调表达。大豆毛状根试验发现,过量表达GmVIT1的毛状根耐受150 mmol·L-1氯化钠;转基因拟南芥耐盐性试验表明GmVIT1可显著提高植物耐盐能力。
Vacuolar iron transporters can maintain the contents of iron in plant cells to ensure the normal growth of cells,which play an important role in enhancing the salt tolerance of plants.GmVIT1,a homologous gene of vacuolar iron transporters,was cloned from soybean.Analysis of the protein domain showed that GmVIT1 belonged to the CCC1 protein superfamily and had a highly conserved domain.The study found that GmVIT1 had a transmembrane structure,and GmVIT1 protein was located on the tonoplast by YFP fluorescent protein;the real-time fluorescent quantitative PCR(qRT-PCR)showed that GmVIT1 was expressed in soybean roots,stems,leaves,flowers,pods and seeds,and the abundance of mRNA in flower was relatively high;it was found that mRNA accumulation of GmVIT1 was negatively regulated by ABA,and geneexpressionwas significantly up-regulated under salt stress.The test of soybean hairy roots found that hairy roots which overexpressed GmVIT1 could tolerate 150 mmol·L-1 sodium chloride;the salt tolerance test of transgenic Arabidopsis thaliana also confirmed that GmVIT1 could significantly improve the salt tolerance of plants.
作者
李永光
景雅
孙铭阳
张沿政
苌兴超
陈龙
李文滨
LI Yongguang;JING Ya;SUN Mingyang;ZHANG Yanzheng;CHANG Xingchao;CHEN Long;LI Wenbin(School of Agriculture,Northeast Agricultural University,Harbin 150030,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2020年第2期10-18,共9页
Journal of Northeast Agricultural University
基金
转基因生物新品种培育科技重大专项(2016ZX08004002)。
关键词
大豆
拟南芥
耐盐
液泡铁离子转运
soybean
Arabidopsis thaliana
salt tolerance
vacuolar iron transporters
