摘要
目的测定金钗石斛居群内不同丛和不同年生枝条中石斛碱的含量,并对其进行比较与分析。方法采用气相色谱法,DB-1毛细管柱(30 m×0.25 mm×0.25μm),火焰离子化检测仪(FID)为检测器测定不同丛和不同年生枝条石斛碱的含量。结果金钗石斛居群内不同丛和不同年生枝条中石斛碱含量均有差异,最高含量为0.94%,最低含量为0.13%。生长年限是影响同一丛中不同枝条石斛碱含量的主要因素,1年生>2年生>3年生。在生长年限相同的条件下,地势是石斛碱含量差异的主要因素,高地势金钗石斛中石斛碱含量显著高于低地势。结论金钗石斛居群内样本中的石斛碱含量差异显著,可以为金钗石斛的种植、采收提供参考。
Objective To analyze the contents of dendrobine in different clumps and branches of Dendrobium nobile within the population.Methods The contents of dendrobine in different clumps and branches of Dendrobium nobile were determined using Gas chromatography with DB-1 capillary column(30 m×0.25 mm×0.25μm)and flame ionization detector(FID).Results The dendrobine contents in different clumps and branches of the population were various,with the highest content reaching 0.94%and the lowest content being 0.13%.The growth year was negatively correlated with the dendrobine contents of different stems in the same clump.The order was annual,two years and three years.Conclusion The dendrobium contents in the samples of the population were significantly different,which can provide a reference for the cultivation and harvest of Dendrobium nobile.
作者
陆安静
余丹丹
何慧雨
杜艺玫
张倩茹
白朝钧
杨洲
杨继勇
谭道鹏
何芋岐
Lu Anjing;Yu Dandan;He Huiyu;Du Yimei;Zhang Qianru;Bai Chaojun;Yang Zhou;Yang Jiyong;Tan Daopeng;He Yuqi(Key Lab of the Basic Pharmacology of the Ministry of Education and Joint International Research Laboratory of Ethnomedieine of Ministry of Education,School of Pharmacy,Zunyi Medical University,Zunyi Guizhou 563099,China;Guizhou Nadier Biotech Co.,Ltd.,Guiyang Guizhou 550009,China;Guangzhou Linghan Pharmaceutical Technology Co.,Ltd.,Guangzhou Guangdong 510080,China;Shanghai Shi Dande Standard Technical Service Co.,Ltd.,Shanghai 201203,China;Chishui Xintian Chinese Medicine Industry Development Co,Ltd,Chishui Guizhou 564700,China)
出处
《遵义医科大学学报》
2020年第1期41-46,共6页
Journal of Zunyi Medical University
基金
国家自然科学基金资助项目(NO:81660685)
贵州省科技支撑计划项目(NO:黔科合支撑[2019]2961,黔科合支撑[2019]2953,黔科合支撑[2020]4Y072)
贵州省优秀青年科技人才项目(NO:黔科合平台人才[2019]5657)
贵州省科技重大专项(NO:黔科合重大专项字[2019]3001,黔科合重大专项字[2015]6010)
遵义市科技计划项目(NO:遵市科合HZ字(2019)3)
贵州石斛产业发展研究中心项目(NO:黔石科合[2019003])。
关键词
金钗石斛
石斛碱
气相色谱法
居群
Dendrobium nobile
Dendrobine
gas chromatography
population
