摘要
目的研究天然抗菌剂对金黄色葡萄球菌、大肠杆菌、绿脓杆菌、白念珠菌和黑曲霉的抑制作用。方法采用杯碟法和微孔板法测定天然抗菌剂对5种致病菌的抑菌圈大小、最小抑菌浓度(MIC)及对10株金黄色葡萄球菌的MIC。结果1%天然抑菌剂对金黄色葡萄球菌、大肠杆菌、绿脓杆菌、白念珠菌和黑曲霉的抑菌圈分别是14.47 mm、10.67 mm、9.13 mm、28.13 mm、10.53 mm;天然抑菌剂对金黄色葡萄球菌、大肠杆菌、绿脓杆菌、白念珠菌和黑曲霉的MIC分别是0.08%、0.16%、0.63%、0.08%、0.16%,阳性对照(Sensiva SC50)对这5种菌的MIC分别是0.16%、0.16%、0.31%、0.16%、0.13%;天然抗菌的对10株金黄色葡萄球菌的MIC值分别为0.08%、0.06%、0.05%、0.09%、0.06%、0.07%、0.10%、0.06%、0.09%、0.07%。结论天然抗菌剂对这5种菌均有一定的抑制作用,对金黄色葡萄球菌和白念珠菌的抑制效果优于阳性对照,对大肠杆菌和黑曲霉的抑制效果与阳性药相当,对绿脓杆菌的效果略差于阳性药,对10株金黄色葡萄球菌均有较好的抑制作用。
Objective To study the inhibitory effects of natural antibacterial agent on Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Candida albicans and Aspergillus niger.Methods The diameter of inhibition zoon and the minimum inhibitory concentration(MIC)of natural antibacterial agent against 5 pathogenic microorganisms and MICs of 10 strains of Staphylococcus aureus were determined by cup and dish method,microporous plate method.Result The diameter of inhibition zoon of 1%natural antibacterial agent against S.aureus,E.coli,P.aeruginosa,C.albicans and A.niger were 14.47mm,10.67mm,9.13mm,28.13mm and 10.53mm,respectively.The MICs of natural antibacterial agents against S.aureus,E.coli,P.aeruginosa,C.albicans and A.nigerwere 0.08%,0.16%,0.63%,0.08%and 0.16%,respectively.The MICs of Sensiva SC50 against these five microorganisms were 0.16%,0.16%,0.31%,0.16%and 0.13%,respectively.The MICs of 10 strains of S.aureus were 0.08%、0.06%、0.05%、0.09%、0.06%、0.07%、0.10%、0.06%、0.09%、0.07%,respectively.Conclusion The natural antibacterial agent had a certain inhibitory effect on the five microorganisms,the inhibitory effects on S.aureus and C.albicans were better than the positive control,the inhibitory effects on E.coli,and A.niger were similar to the positive control,and the effect on P.aeruginosa was slightly worse than the positive control.And all the 10 stains of S.aureus showed good inhibitory effect.
作者
刘光荣
翟春涛
金敏蓉
李成亮
LIU Guang-rong;ZHAI Chun-tao;JIN Min-rong;LI Cheng-liang(Infinitus(China)Company Ltd,Guangzhou 510663,China;LB Cosmeceutical Technology Co.,Ltd.,shanghai 201401,China)
出处
《中国真菌学杂志》
CSCD
2020年第2期88-92,共5页
Chinese Journal of Mycology
关键词
天然抗菌剂
金黄色葡萄球菌
大肠杆菌
绿脓杆菌
白念珠菌
黑曲霉
抑菌圈
最小抑菌浓度
natural antibacterial agent
Staphylococcus aureus
Escherichia coli
Pseudomonas aeruginosa
Candida albicans
Aspergillus niger
inhibition zoon
MIC
