摘要
探究瞬时受体电位阳离子通道TRPA1/TRPV1在COPD发生机制中的作用。通过检测对照组正常肺组织和实验组COPD患者肺组织中TRPA1,TRPV1,IL-8和IL-18的mRNA的表达水平,两组肺组织中TRPA1,TRPV1蛋白表达量的变化情况来研究TRPA1/TRPV1在COPD发生机制中的作用。通过荧光定量PCR的结果分析显示,相比对照组的正常肺组织中TRPA1,TRPV1,IL-8,IL-184个基因的表达量COPD组的基因表达量都有显著性增加,其中TRPA1的表达量上调21倍,TRPV1的表达量上调15倍,IL-8和IL-18的表达量分别上调了12倍和9倍,统计均具有极显著性意义,P<0.01。用Western blot的方法检测蛋白质的表达量结果显示,COPD组的TRPA1和TRPV1和正常的对照组相比较,蛋白质的表达量升高,P<0.05。结果显示,瞬时受体电位阳离子通道TRPA1/TRPV1在COPD发生中通过相互作用调节肺部感觉神经元的兴奋性,并且通过调节IL-8和IL-18的表达参与其气道炎症反应。
To explore the role of transient receptor potential cation channel TRPA1/TRPV1 in the mechanism of COPD development,the expression levels of TRPA1,TRPV1,IL-8 and IL-18 in the lung tissue of normal lung tissue and experimental COPD patients in the control group and the expression of TRPA1 and TRPV1 in lung tissue of the two groups were studied to study the occurrence of TRPA1/TRPV1 in COPD and the role of the mechanism.The results of quantitative analysis showed that the expression levels of TRPA1,TRPV1,IL-8,and IL-18 in the normal lung tissues of the control group were significantly increased in the COPD group,and the expression of TRPA1 was up-regulated by 21 times.The expression level of TRPV1 was up-regulated by 15 times,and the expression levels of IL-8 and IL-18 were up-regulated by 12-fold and 9-fold,respectively.The statistics were extremely significant,P<0.01.The expression of protein by Western blot showed that the expression of TRPA1 and TRPV1 in the COPD group was higher than that in the normal control group,P<0.05.In summary,the transient receptor potential cation channel TRPA1/TRPV1 regulates the excitability of pulmonary sensory neurons through interaction in the development of COPD,and participates in its airway inflammation by regulating the expression of IL-8 and IL-18.
作者
刘亚男
李晓佳
金雪梅
江德鹏
LIU Ya-nan;LI Xiao-jia;JIN Xue-mei;JIANG De-peng(Department of Respiratory Medicine,People's Hospital of Jiulongpo District,Chongqing 400000,China;Department of Respiratory Medicine,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)
出处
《药物生物技术》
CAS
2020年第1期38-41,共4页
Pharmaceutical Biotechnology
