摘要
目的考察禾本科芦笋中总黄酮在D101大孔吸附树脂上静态与动态吸附性能,优选总黄酮纯化工艺。方法以静态吸附率、解吸率、静态吸附曲线为依据比较D101、AB-8和HPD100的静态吸附性能,优选最优树脂,然后对其动态吸附工艺进行考察。结果静态吸附量大小关系为AB-8>D101>HPD100,采用95%乙醇解吸附时解吸率大小顺序为HPD100>AB-8>D101。以D101大孔树脂进行动态吸附观察,动态优选的结果为5床容积(BV)的样品(水∶样品的容积比为1∶0.25)上样于径高比1∶5的D101大孔树脂柱,静置4 h,7 BV水洗,60 ml 90%乙醇洗脱,纯度提高了6.54倍。结论D101大孔吸附树脂可以用于禾本科芦笋中总黄酮的纯化。
Objective To investigate the static and dynamic adsorption properties of total flavonoids in Asparagus of Gramineae on D101 macroporous resins and optimize the purification process for total flavonoids.Methods The static adsorption rate,desorption rate and static adsorption curve were determined to compare the static adsorption performance of D101,AB-8 and HPD100,and the best resin was selected optimally.Then the dynamic adsorption process was investigated.Results The relationship of static adsorption capacity was AB-8>D101>HPD100,and the order of desorption rate was HPD100>AB-8>D101 by using 95%alcohol for desorption.The dynamic adsorption was investigated by using D101 macroporous resin.The result of dynamic optimization was that 5 bed volume(BV)of the sample(the volume ratio of water to sample was 1︰0.25)was placed on D101 macroporous resin column that its ratio of diameter to height was 1︰5.After the sample sat quietly for 4 hours,it was washed with 7 BV of water and 60 ml of 90%ethanol,then the purity was increased by 6.54 times.Conclusion D101 macroporous resin may be used for purifying the total flavonoids in Asparagus of Gramineae.
作者
谢稳
雷雅婷
张也
罗堃
王元清
蒋波
严建业
Xie Wen;Lei Yating;Zhang Ye;Luo Kun;Wang Yuanqing;Jiang Bo;Yan Jianye(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Center of Innovative Technology,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Yongzhou Hospital of Traditional Chinese Medicine,Yongzhou 425000,Hunan,China;Biotechnology and Engineering Laboratory,College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,Hunan,China;Hunan Center for Drug Evaluation,Certification and ADR Monitoring,Changsha 410013,Hunan,China)
出处
《右江民族医学院学报》
2020年第2期163-166,174,共5页
Journal of Youjiang Medical University for Nationalities
基金
长沙市科技计划项目(kq1701070)
湖南中医药大学与沅江市政府科研合作基金项目[科函(2016)6号]
湖南中医药大学一层次中药学学科资助(湘教通〔2011〕76号)。
关键词
芦笋
D101大孔树脂
总黄酮
静态
动态
Asparagus
D101 macroporous resin
total flavonoids
static state
dynamic state
