摘要
目的探讨激活转录因子-2(activating transcription factor-2,ATF-2)在非特指型弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,not otherwise specified,DLBCL,NOS)中表达的意义,并分析其表达与DLBCL,NOS的临床病理特征及预后之间的关系。方法收集60例DLBCL,NOS病例及40例淋巴结反应性增生(reactive hyperplasia,RH)病例的临床病理资料,采用免疫组织化学染色方法(MAX Vision法)检测DLBCL,NOS肿瘤细胞中ATF-2、CyclinD1、BCL-2、CD10、BCL-6及MUM1蛋白的表达情况和ATF-2蛋白在淋巴结RH中的表达。比较ATF-2蛋白在DLBCL,NOS和淋巴结RH两组病例中的表达,并分析ATF-2蛋白表达与DLBCL,NOS的Hans分型、BCL-2、CyclinD1蛋白表达及其他临床病理特征和预后之间的关系。结果ATF-2蛋白在DLBCL,NOS与淋巴结RH组织中均呈现为细胞核染色,染色程度强弱不等,两组病例中,ATF-2蛋白表达的差异有统计学意义(P=0.000)。ATF-2蛋白在DLBCL,NOS不同年龄、性别及Hans分型分组中表达的差异无统计学意义(P>0.05)。ATF-2蛋白在DLBCL,NOS中的表达与CyclinD1及BCL-2蛋白的表达无相关性(P>0.05)。DLBCL,NOS病例中ATF-2蛋白高表达患者与低表达患者总生存期的差异无统计学意义(P=0.194)。结论ATF-2蛋白在DLBCL,NOS中的表达明显高于在淋巴结RH中的表达,因此推测ATF-2蛋白核内高表达与DLBCL,NOS的发生发展有一定关系,可为研究其发病机制、临床治疗提供一条新的思路。
Objective To investigate the significance of activating transcription factor-2(ATF-2)in diffuse large B-cell lymphoma,not otherwise specified(DLBCL,NOS),and to analyze its correlation with clinicopathological features.Methods Sixty cases of DLBCL,NOS and 40 cases of lymph node RH were enrolled.The expression of ATF-2,CyclinD1,BCL-2,CD10,BCL-6 and MUM1proteins in DLBCL,NOS and the expression of ATF-2 protein in lymph node RH cases were assessed using immunohistochemistry staining.The correlation between the expression of ATF-2 protein and the prognostic grouping,the expression of BCL-2 and CyclinD1 protein,or the clinicopathological features in DLBCL,NOS cases were analyzed.Results ATF-2 protein expressed in nucleus both in DLBCL,NOS and lymph node RH cases,which showed different staining intensity.The expression of ATF-2 protein was significantly different between the DLBCL,NOS cases and lymph node RH cases(P=0.000).There was no significantly difference in the expression of ATF-2 amogn indifferent ages,genders,or Hans classification of DLBCL,NOS(P>0.05).The expression of ATF-2 was not significantly correlated with the expression of CyclinD1 of BCL-2 in DLBCL,NOS cases(P>0.05).There was no significantly difference in overall survival between the high ATF-2 protein expression and the low ATF-2 protein expression in DLBCL,NOS cases(P=0.194).Conclusion The expression of ATF-2 protein in DLBCL,NOS patients is significantly higher than that in lymph node RH patients.The high expression of ATF-2 protein is correlated to the occurrence and development of DLBCL,NOS.
作者
袁振亚
王明华
翁阳
YUAN Zhen-ya;WANG Ming-hua;WENG Yang(Department of Pathology,the First Affiliated Hospital of Hainan Medical University,Haikou 570100,Hainan,China;不详)
出处
《广东医学》
CAS
2020年第6期575-579,共5页
Guangdong Medical Journal
基金
海南省自然科学基金项目(817327)。
