摘要
通过将大肠杆菌(E.coli)、海藻酸钠(SA)和MIL-53(Fe)掺杂到聚乙烯醇(PVA)胶体中形成纳米复合材料,并与硼酸交联得到MIL-53(Fe)/E.coli/SA/PVA颗粒。以E.coli作为受试体,K3[Fe(CN)6]为媒介体负责转运固定化微生物呼吸作用产生的电子至电极表面,通过监测还原产物([Fe(CN)6]4-)的量的变化,从而得到微生物活性变化的信息,进而衡量药物的有效性。通过筛选MIL-53(Fe)/E.coli/SA/PVA颗粒的最佳形成条件,制备了灵敏的生物探针,搭建了基于固定化微生物的传感体系,并应用于不同抗生素类药物的检测。
Escherichia coli(E.coli),sodium alginate(SA)and MIL-53(Fe)were doped into polyvinyl alcohol(PVA)to form a nanocomposite.As used herein,boric acid was used as a crosslinking agent to prepare MIL-53(Fe)/E.coli/SA/PVA pellets.The quality control strain of E.coli and K3[Fe(CN)6]were taken as tested strain and mediator,respectively.Then K3[Fe(CN)6]was responsible for transporting electrons generated by immobilized microorganism respiration to the electrode surface.In order to measure the efficiencies of the drugs,the changes of microbial activity had to be measured by monitoring the quantity changes of reduced product([Fe(CN)6]4-).The optimal conditions of MIL-53(Fe)/E.coli/SA/PVA were screened to prepare a sensitive biological probe,which was used to building a sensing system based on immobilized microorganisms,for applying in the detection of different antibiotics.
作者
陈立志
韩笑
何迪
贺敬婷
于佳雪
马烨
刘畅
CHEN Li-zhi;HAN Xiao;HE Di;HE Jing-ting;YU Jia-xue;MA Ye;LIU Chang(College of Pharmacy,Jinzhou Medical University,Jinzhou 121000,China)
出处
《化学试剂》
CAS
北大核心
2020年第4期354-358,共5页
Chemical Reagents
基金
辽宁省大学生创新创业训练项目(2015101600-00007)。
