摘要
目的:研究板蓝根多糖(RIP)影响NK细胞对食管癌细胞杀伤作用的分子机制。方法:MTT法检测细胞增殖率,Western blot检测增殖相关蛋白CyclinD1、p21和p27的表达,ELISA法检测培养上清中IFN-γ和TNF-α的表达,流式细胞术检测NKG2D配体MICA、MICB、ULBP1和ULBP2的表达,钙黄绿素释放法(CARE-LASS)检测NK细胞对食管癌细胞的杀伤率。结果:RIP可促进NK-92细胞增殖,抑制食管癌Eca-109细胞增殖,且具有显著剂量依赖性。食管癌可促进NK细胞中TNF-α和IFN-γ的表达。RIP可促进食管癌细胞Eca-109中NKG2D配体MICA、MICB、ULBP1和ULBP2的表达,促进与食管癌细胞联合作用后NK细胞中TNF-α和IFN-γ表达,提高NK-92细胞对食管癌Eca-109细胞的杀伤活性。结论:RIP通过提高食管癌Eca-109细胞中NKG2D配体表达,促进NK-92细胞中TNF-α和IFN-γ表达,增强NK-92细胞对食管癌细胞的杀伤作用。
Objective:To investigate the molecular mechanism of radix isatidis polysaccharide(RIP)on killing effect of NK cells on esophageal cancer cells.Methods:MTT assay was carried out to detect the cell proliferation rate.Western blot were applied to measure the expression levels of proliferation-related proteins CyclinD1,p21 and p27.ELISA was used to detect the secretion of IFN-γand TNF-αin the supernatant.Flow cytometry was applied to detect the expression of NKG2D ligands induding MICA,MICB,ULBP1 and ULBP2.Calcein-releasing assay(CARE-LASS)was implemented to detect the killing rate of NK cells on esophageal cancer cells.Results:RIP promoted the proliferation of NK-92 cells and inhibited the proliferation of Eca-109 cells in a dose-dependent way.Esophageal cancer promoted the expression of IFN-γand TNF-αin NK cells.RIP promoted the expression of NKG2D ligand MICA,MICB,ULBP1 and ULBP2 in esophageal cancer cells Eca-109,promoted the expression levels of IFN-γand TNF-αin NK cells when combined with esophageal cancer cells,and enhanced the killing rate of NK-92 cells on Eca-109 cells.Conclusion:RIP enhances the killing effect of NK-92 cells on esophageal cancer cells by promoting the expression of NKG2D ligand in Eca-109 cells and raising the expression of IFN-γand TNF-αin NK-92 cells.
作者
梁宗英
侯继申
孙光蕊
赵宝山
辛国华
LIANG Zong-Ying;HOU Ji-Shen;SUN Guang-Rui;ZHAO Bao-Shan;XIN Guo-Hua(Department of Thoracic Surgery,Affiliated Hospital of Chengde Medical College,Chengde 067000,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2020年第8期965-970,共6页
Chinese Journal of Immunology
关键词
食管癌
NK细胞
板蓝根多糖
NKG2D配体
杀伤作用
Esophageal cancer
NK cell
Radix isatidisa polysaccharide(RIP)
NKG2D ligand
Killing effect
