摘要
香合欢具有较好的经济价值,但由于种子硬实度较高,无法满足推广、利用的需求。本试验以香合欢成熟种子为材料,进行硬实破除及丛芽诱导研究。试验方法为:①采用热水及98%浓硫酸进行硬实破除;②将切除子叶及大部分下胚轴后2 cm长的芽,接种到培养基上进行丛芽诱导。主要研究结果如下:①热水处理最佳的硬实破除方式为85℃热水浸种24 h,发芽率和发芽势分别为80.00%和95.56%;98%浓硫酸处理较好的硬实破除方式为酸蚀10~30 min,发芽率和发芽势均达到98.89%~100.00%;②最佳丛芽诱导培养基为MS+6-BA1.0 mg/L+NAA0.3 mg/L。结论:①种皮是造成香合欢种子休眠,萌发率较低的主要原因,而胚本身不含有影响其萌动的物质。②香合欢属于较易于诱导丛芽的一种木本植物。
Albizia odoratissima(L.f.) Benth has good economic value. However, due to the higher hardness of seeds, it is unable to meet the demands of development and utilization.In this experiment, the mature seeds of Albizia odoratissima were broken open and buds were induced. Method: ① Seeds were broken open with hot water and 98 % concentrated sulfuric acid. ② Cluster buds were induced from cotyledon and 2 cm long buds of hypocotyl on the medium. Results:①The optimum conditions of hot water treatment were as follows: soaking temperature 85℃, soaking time 24 h, under which the germination rate and germination potential were 80.00% and 95.56 %, respectively;treating with 98 % concentrated sulfuric acid for 10~30 min was the best, and the germination rate and germination potential reached 98.89 %~100.00 %. ② The optimal medium for shoot induction was MS+ 6-BA1.0 mg/L + NAA0.3 mg/L.Conclusion: ① Hard seed coat causes dormancy and low germination rate, but embryo does not contain substances affecting germination. ②Albizia odoratissima(L.f.) Benth is a woody plant that is easy to induce shoots.
作者
许倩
林纬
黎起秦
袁高庆
赖家业
XU Qian;LIN Wei;LI Qiqin;YUAN Gaoqing;LAI Jiaye(Forestry College,Guangxi University,Nanning 530000,China;Agricultural and Animal Husbandry Industry Development Research Institute,Guangxi University,Nanning 530000,China;Agricultural College,Guangxi University,Nanning 530000,China;Key and Open Laboratory of Central South Fast-growing Timber Breeding,State Forestry Administration,Nanning,Guangxi 530000,China)
出处
《农业研究与应用》
2020年第1期16-21,共6页
Agricultural Research and Application
基金
广西科技重大专项(桂科AA17204058-17)。
关键词
香合欢
硬实破除
组织培养
丛芽诱导
Albizia odoratissima(L.f.) Benth
seed coat breaking
tissue culture
shoot induction
