摘要
研究防风不同极性部位指纹图谱与抗流感病毒活性的谱效关系,推断防风中具有抗流感病毒活性的有效成分。本实验采用细胞病变效应(cytopathic effect,CPE)法检测防风不同极性部位的抗流感病毒活性;建立防风不同极性部位的UPLC-Q-Exactive Orbitrap/MS指纹图谱;采用Spearman分析、灰色关联度分析(GRA)及偏最小二乘回归(PLSR)分析法分析防风不同极性部位的UPLC特征指纹峰与其抗流感病毒活性之间的谱效关系。在标定的28个特征指纹峰中,确定了1、5、7、11、13、20、24号峰与抗流感病毒活性呈正相关,其对应的物质依次是焦谷氨酸、divaricatacid、升麻苷、亥茅酚苷、补骨脂素、异嗪皮啶、5-羟基-8-甲氧基补骨脂素,为深入研究防风抗流感病毒活性物质基础提供实验基础。
The spectral-effect relationship between the fingerprints of different polar parts of Saposhnikovia divaricata and the anti-influenza virus activity was investigated,and the effective components of S.divaricata with anti-influenza virus activity were deduced.In this study,the cytopathic effect(CPE)method was used to detect the anti-influenza virus activity of different polar parts of S.divaricata,and the UPLC-Q-Exactive Orbitrap/MS fingerprints of different polar parts of S.divaricata were established;Spearman analysis,gray correlation analysis(GRA)and partial least squares regression(PLSR)analysis were adopted to analyze the spectral-effect relationship between the characteristic fingerprint peaks of UPLC and its anti-influenza virus activity.Among the 28 characteristic fingerprint peaks of calibration,peaks 1,5,7,11,13,20 and 24 were positively correlated with the anti-influenza virus activity,and the corresponding constituents were pyroglutamic acid,divaricatacid,prim-o-glucosylcimifugin,sec-o-glucosylhamaudol,psoralen,isofraxidin and 5-hydroxy-8-methoxypsoralen.The collective results will provide experimental basis for in-depth research on the active substance basis of anti-influenza virus of S.divaricata.
作者
姜晓琳
张继秋
徐瑞蕊
蒋明浩
亢倩丽
杨勇
巩丽丽
JIANG Xiao-lin;ZHANG Ji-qiu;XU Rui-rui;JIANG Ming-hao;KANG Qian-li;YANG Yong;GONG Li-li(School of Pharmacy,Shandong University of Traditional Chinese Medicine;Experience Center of Shandong University of Traditional Chinese Medicine,Jinan 250355,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第3期473-481,397,共10页
Natural Product Research and Development
基金
国家自然科学基金面上项目(81774167)
山东中医药大学2019年大学生创新训练计划(S201910441036)。
