摘要
目的研究宫颈病变患者转录因子GATA-3、T-bet与TH细胞因子的相关性及其在外周血中的表达水平及意义。方法选择2017年1月~2019年1月新疆医科大学第一附属医院就诊的76例患者,根据宫颈液基细胞学检测分为对照组(33例)、低度鳞状上皮内病变(L-SIL)组(10例)及高度鳞状上皮内病变(H-SIL)组(33例)。Rt-PCR检测血清GATA-3及T-bet表达量,流式细胞学检测三组血清TH细胞因子含量,相关性分析采用Pearson相关分析。结果H-SIL组、L-SIL组白细胞介素(IL)-4、IL-10表达水平高于对照组,GATA-3表达量高于对照组,L-SIL组T-bet表达量低于对照组,差异均有统计学意义(均P<0.05)。IFN-γ与T-bet呈正相关(r=0.65,P<0.05),与GATA-3呈负相关(r=-0.51,P<0.05);IL-4与GATA-3呈正相关(r=0.56,P<0.05),与T-bet呈负相关(r=-0.72,P<0.05);IL-10与T-bet呈负相关(r=-0.65,P<0.05)。结论L-SIL患者的外周血中表现为TH1细胞优势,T-bet的基因表达上调,而H-SIL患者表现为TH2细胞优势,T-bet的基因表达下调。可见,L-SIL、H-SIL的发生与TH细胞因子、GATA及T-bet具有相关性。
Objective To study the correction between transcription factors GATA-3,T-bet and TH cytokine in patients with cervical lesions and their expression level and significance in peripheral blood.Methods A total of 76 patients who were admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2017 to January 2019 were selected.According to the cervical thinprep cytologic test,they were divided into control group(33 cases),low-grade squamous intraepithelial lesion(L-SIL)group(10 cases)and high-grade squamous intraepithelial lesion(H-SIL)group(33 cases).The expression levels of GATA-3 and T-bet in serum were detected by Rt-PCR,and the TH cytokine levels in serum of three groups were detected by flow cytometry.Pearson correlation analysis was used for correlation analysis.Results The expressions of interleukin(IL)-4 and IL-10 in H-SIL group and L-SIL group were higher than those in control group,and the expression of GATA-3 was higher than that in control group,while the expression of T-bet in L-SIL group was lower than that in control group,and the differences were statistically significant(all P<0.05).IFN-γwas positively correlated with T-bet(r=0.65,P<0.05),and negatively correlated with GATA-3(r=-0.51,P<0.05).IL-4 was positively correlated with GATA-3(r=0.56,P<0.05),and negatively correlated with T-bet(r=-0.72,P<0.05).IL-10 was negatively correlated with T-bet(r=-0.65,P<0.05).Conclusion Peripheral blood of patients with L-SIL shows dominance of TH1 cells and up-regulated T-bet gene expression,while that of patients of H-SIL shows dominance of TH2 cells and down-regulated T-bet gene expression.It can be seen that the occurrence of L-SIL and H-SIL is related to TH cytokines,GATA and T-bet.
作者
赛星月
李晓红
杜蓉
SAI Xingyue;LI xiaohong;DU Rong(Department of Gynaecology,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830054,China)
出处
《中国医药导报》
CAS
2020年第11期76-79,共4页
China Medical Herald
基金
国家自然科学基金资助项目(81660476)。
关键词
细胞因子
转录因子
宫颈病变
Cytokines
Transcription factors
Cervical lesions
