摘要
孔雀石绿作为一种化学制剂,因其杀菌性较好、价格便宜以及少量添加不易察觉等特点,一些商家将其作为杀菌剂在水产品中使用。然而,因孔雀石绿代谢产物隐性孔雀石绿具有稳定性以及高毒性的化学性质,其已被我国列为禁用药物。该研究深入讨论了如何使用三维荧光光谱法检测水产品中的隐性孔雀石绿,利用三维荧光光谱技术,探究隐性孔雀石绿的三维特征,并进一步分析其荧光强度与pH变化关系,以研究其最佳检测环境。最后针对鱼肉样品进行荧光光谱检测,以讨论三维荧光光谱技术的可行性。为三维荧光光谱法检测水产品中隐性孔雀石绿进行积累数据和奠定基础。经初步实验可以得出:隐性孔雀石绿乙醇溶液荧光强度最高峰值在激发波长324 nm/发射波长355 nm处,隐性孔雀石绿甲醇溶液荧光强度最高峰值在激发波长320 nm/发射波长355 nm处;且在pH 7的环境中检测效果最为明显。针对鱼肉样品的检测结果表明:隐性孔雀石绿浓度和荧光强度呈良好线性关系,经计算得到加标回收率为96.65%,证明实验结果有较好的稳定性。因此水产品中的隐性孔雀石绿可以通过三维荧光光谱技术准确快速的进行检测。
As a chemical agent,malachite green is used as a fungicide in aquatic products because of its good bactericidal properties,low price and a small amount are imperceptible.However,because its metabolite in the body--the recessive malachite green has stability and highly toxic chemical properties.Malachite green has been classified as a banned drug in China.This experiment discusses how to use three-dimensional fluorescence spectroscopy to detect recessive malachite green in aquatic products,and use three-dimensional fluorescence spectroscopy to explore the three-dimensional characteristics of recessive malachite green,and further analyze the relationship between fluorescence intensity and pH change.Finally,fluorescence spectroscopy is carried out for fish samples to discuss the feasibility of three-dimensional fluorescence spectroscopy.The accumulation data and foundation of recessive malachite green in aquatic products are detected by three-dimensional fluorescence spectrometry.The experimental results show thatthe highest peak of the recessive malachite green ethanol solution was obtained at the excitation wavelength of 324 nm/the emission wavelength of 355 nm.The highest peak of recessive malachite green methanol solution was obtained at the excitation wavelength of 320 nm/the emission wavelength of 355 nm.The experimental results show that the fluorescence peak strength is strongest in the pH 7.0 environment.According to the experience of fish sample,we can find that the relationship between the concentration of recessive malachite green and the fluorescence intensity showed a good linear relationship.The recovery rate was 96.65%,which proved that the experimental results have good stability.Therefore,the recessive malachite green in aquatic products can be accurately and quickly detected by three-dimensional fluorescence spectroscopy technology.
作者
李宁驰
任诺钰
刘春宇
姚治海
蔡红星
LI Ning-chi;REN Nuo-yu;LIU Chun-yu;YAO Zhi-hai;CAI Hong-xing(Provincial Key Laboratory of Spectral Detection Science and Technology,College of Science and Technology,Changchun Uni-versity of Science and Technology,Changchun 130012,China;Jilin Qiushi Spectral Data Technology Co.,Ltd.,Changchun 130012,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2020年第5期1478-1482,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(11505017,61405189)
吉林省教育厅“十三五”科学技术项目(JJKH20190553KJ)
吉林省高教科研课题(JGJX2019D61)资助。
关键词
三维荧光光谱
隐性孔雀石绿
水产品
鱼肉检测
Three-dimensional fluorescence spectrum
Recessive malachite green
Aquatic products
Fish detection
