摘要
目的探究CD3^+HLA-DR细胞与早发性卵巢功能不全(POI)患者免疫紊乱、卵巢功能的关系。方法选取本院2018年1月至2019年9月POI患者136例作为观察组,另选取同期健康育龄女性130例作为对照组。检测对比两组外周血CD3^+HLA-DR细胞比例、免疫球蛋白A(IgA)、IgM、IgG、细胞免疫(CD3^+、CD8^+、CD4^+)、血清抗苗勒氏管激素(AMH)、基础窦状卵泡数(AFC)、子宫内膜厚度,采用Pearson相关性分析外周血CD3^+HLA-DR细胞与免疫指标、卵巢功能的关系。结果观察组外周血CD3^+HLA-DR细胞比例高于对照组,差异有统计学意义(P<0.05);观察组CD4^+、血清IgA、IgM、IgG水平低于对照组,CD3^+、CD8^+水平高于对照组,差异有统计学意义(P<0.05);外周血CD3^+HLA-DR细胞与CD4^+、血清IgA、IgM、IgG呈负相关,与CD3^+、CD8^+呈正相关(P<0.05);观察组血清AMH、AFC、子宫内膜厚度低于对照组,差异有统计学意义(P<0.05);外周血CD3^+HLA-DR细胞与血清AMH、AFC、子宫内膜厚度呈负相关(P<0.05)。结论外周血CD3^+HLA-DR细胞在POI患者中呈异常升高表达,并与POI患者免疫紊乱、卵巢功能存在一定相关性,有望成为调控POI患者免疫应答、改善卵巢储备功能的重要T细胞亚群。
Objective To investigate the relationship between CD3^+HLA-DR cells and immune disorders and ovarian function in patients with early-onset ovarian insufficiency(POI). Methods From January 2018 to September 2019,136 patients with POI in our hospital were selected as the observation group,and130 healthy women of childbearing age during the same period were selected as the control group. The ratio of peripheral blood CD3^+HLA-DR cells,immunoglobulin A(IgA),IgM,IgG,cellular immunity(CD3^+,CD8^+,CD4^+),serum anti-Mullerian hormone(AMH),basal sinus follicle number(AFC),endometrial thickness was compared between the two groups,Pearson correlation was used to analyze the relationship between peripheral blood CD3^+HLA-DR cells and immune indicators and ovarian function. Results The proportion of peripheral blood CD3^+HLA-DR cells in the observation group was higher than that in the control group,and the difference was statistically significant(P<0.05). The levels of CD4^+,serum IgA,IgM,and IgG in the observation group were lower than those in the control group,and the levels of CD3^+and CD8^+were higher than those in the control group(P<0.05). CD3^+HLA-DR cells in peripheral blood were negatively correlated with CD4^+,serum IgA,IgM,and IgG,and positively correlated with CD3^+and CD8^+(P<0.05). The AMH,AFC,and endometrial thickness of the observation group were lower than those of the control group(P<0.05). CD3^+HLA-DR cells in peripheral blood were negatively correlated with serum AMH,AFC,and endometrial thickness(P<0.05). Conclusion Peripheral blood CD3^+HLA-DR cells are abnormally elevated expression in patients with POI,and have a certain correlation with immune disorders and ovarian function in patients with POI. It is expected to become an important T cell subset that regulates the immune response of POI patients and improves ovarian reserve function.
作者
郭蜜甜
张继
王素梅
GUO Mitian;ZHANG Ji;WANG Sumei(Department of obstetrics and gynecology,zhengzhou 7th people's hospital,Zhengzhou Henan,China,450016)
出处
《分子诊断与治疗杂志》
2020年第3期340-344,共5页
Journal of Molecular Diagnostics and Therapy
基金
河南科技厅重点项目(20190607X)。
