摘要
目的:建立地榆槐角丸HPLC指纹图谱,测定其中7个有效成分含量。方法:采用HPLC法,以甲醇(A)-0.05%磷酸水溶液(B)为流动相梯度洗脱,流速为1.0 mL·min-1,检测波长278 nm,柱温35℃,进样量10μL,通过相似度评价对14批地榆槐角丸指纹图谱进行质量评价,并对其中的7个指标成分进行定量分析。结果:在指纹图谱研究中,标定了20个共有峰,通过与对照品比较指认其中7个共有峰分别为没食子酸、芍药苷、柚皮苷、芦丁、槐角苷、黄芩苷和升麻素苷,14批地榆槐角丸与对照图谱的相似度均在0.90以上。结论:所建立的地榆槐角丸指纹图谱和含量测定分析方法可用于地榆槐角丸的质量控制。
Objective:To establish the HPLC fingerprints of Diyu Huaijiao pills and determine the contents of seven compounds. Methods:HPLC analysis was performed with methanol(A)-0.05% phosphoric acid aqueous solution(B)as the mobile phase at a flow rate of 1.0 mL·min-1. The detection wavelength was 278 nm and column temperature was 35 ℃. The injection volume was 10 μL. Similarity evaluation was used to evaluate the fingerprints of 14 batches of Diyu Huaijiao pills and seven markers were quantified. Results:The common mode of the fingerprint showed 20 common peaks,7 of which were identified by comparison with the reference substances. They were gallic acid,paeoniflorin,naringin,rutin,sophoricoside,baicalin and prim-O-glucosylcimifugin. The similarities of 14 batches of samples were above 0.90. Conclusion:The established method can be used for the quality control of Diyu Huaijiao pills.
作者
许雯
赵龙
刘莹
刘耀阳
孙晶
XU Wen;ZHAO Long;LIU Ying;LIU Yao-yang;SUN Jing(The Second Hospital of Shandong University Drug Dispensing Division,Jinan 250031,China;Hunan Xiangtan Institute for Food and Drug Control,Xiangtan 411100,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2020年第3期544-553,共10页
Chinese Journal of Pharmaceutical Analysis
关键词
地榆槐角丸
没食子酸
芍药苷
柚皮苷
芦丁
槐角苷
黄芩苷
升麻素苷
相似度分析
指纹图谱
Diyu Huaijiao pills
gallic acid
paeoniflorin
naringin
rutin
sophoricoside
baicalin
prim-Oglucosylcimifugin
similarity evaluation
fingerprint
