摘要
本研究以小桐子幼苗为材料,在水培条件下,研究了外源水杨酸(SA)对PEG 6000胁迫下小桐子幼苗甘氨酸甜菜碱含量、代谢关键酶BADH活性和相关基因表达,以及Ca2+和CaM抑制剂对SA诱导甜菜碱积累的影响。结果表明:外源1.5 mmol/L SA处理可显著提高渗透胁迫下小桐子幼苗的甜菜碱含量、增加甜菜碱合成关键酶BADH的活性,以及上调JcCMO和JcBADH的表达水平。与单独用PEG处理的幼苗相比,SA处理也提高了渗透胁迫下小桐子幼苗的钙调素(CaM)活性。此外,CaCl2处理能增强SA诱导的甜菜碱积累效应,提高BADH的活性和上调JcBADH的表达水平,而Ca2+通道阻断剂LaCl3、CaM抑制剂CPZ和TFP处理得到相反的结果。这些结果表明,外源SA处理可提高渗透胁迫下小桐子幼苗甜菜碱的生物合成能力,且SA诱导的甜菜碱积累过程可能受到Ca2+/CaM信号的调控。
Hydroponic experiments were carried out to study the effect of exogenous salicylic acid(SA) on the content of glycine betaine(GB), the activity of the key enzyme betaine aldehyde dehydrogenase(BADH) of GB biosynthesis, and the expression of GB metabolism-related genes in the leaves of Jatropha curcas seedlings under PEG 6000 stress. Meanwhile, the effects of Ca2+ and calmodulin(CaM) antagonists on SA-induced GB accumulation were investigated too. SA treatment significantly enhanced the content of GB, increased the activity of BADH, and improved the expression level of JcCMO and JcBADH in the leaves of J. curcas seedlings under osmotic stress. Interestingly, compared to the seedlings exposed to PEG treatment alone, SA treatment significantly increased the activity of CaM under osmotic stress. Calcium chloride(CaCl2) treatment could promote SA-induced GB accumulation. It also induced an increase of BADH activity, and up-regulated JcBADH expression. The results indicate that exogenous SA treatment can enhance the biosynthesis of GB, and Ca2+/CaM signaling might be involved in the regulation of SA-induced GB accumulation.
作者
杨双龙
杨婷
龚明
YANG Shuanglong;YANG Ting;GONG Ming(College of Life Sciences/Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education/Key Laboratory of Biomass Energy and Environmental Biotechnology,Yunnan Normal University,Kunming,Yunnan 650500,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第5期939-946,共8页
Chinese Journal of Tropical Crops
基金
国家自然科学基金(No.31260169,No.31860062)。
