摘要
目的:探讨Morris水迷宫不同逃避潜伏期阈值的设定是否影响阿尔茨海默病(Alzheimer’s disease,AD)转基因小鼠及其同窝同龄野生型(Littermates,LM)小鼠学习和记忆能力的检测结果。方法:将雄性AD转基因鼠与雌性C57BL/6J野生型(Wild-Type,WT)鼠按1:4比例合笼繁育,获得子代小鼠,饲养至3周后进行AD基因表型鉴定。继而选取4月龄雌性AD转基因阳性小鼠17只为AD组,同窝同龄LM雌性小鼠15只为LM组,分析将逃避潜伏期阈值分别设定为90 s和60 s时,AD组和LM组采用Morris水迷宫检测小鼠学习和记忆能力障碍的检出率和可靠性。结果:60 s到90 s期间,AD组小鼠跨越平台次数占比中位数50.0%(0.0%,75.0%),明显高于LM组小鼠跨越平台次数占比中位数16.7%(0.0%,28.6%)(P<0.05);60 s到90 s期间,AD组小鼠目的象限停留时间占比中位数45.2%(37.6%,52.8%),显著高于LM组小鼠目的象限停留时间占比中位数31.7%(28.7%,40.9%)(P<0.05)。AD转基因鼠在潜伏期阈值设定为60 s时较设定为90 s时将损失更多客观实验信息。结论:应用Morris水迷宫检测认知功能障碍时,将逃避潜伏期阈值设定为90 s相较于60 s更能反映出AD鼠空间学习记忆能力的受损程度。
Objective: To investigate whether the threshold setting of different escape latencies of Morris water maze affects the cognitive behavior of Alzheimer’s disease(AD) transgenic mice and their littermate(LM) mice. Methods: Two-month-old male AD mice were mated with three-month-old C57 BL/6 J Wild-Type(WT)female mice in a 1:4 ratio to obtain progeny mice. When the newborn mice were raised to 3 weeks, they were identified for AD gene phenotype. In progeny mice, four-month-old female mice were selected. 17 female AD transgenic positive mice were selected as AD group, while 15 female LM mice were selected as LM group. The detection ability and reliability of cognitive impairment in AD group and LM group were tested by Morris water maze when the threshold of escape latency set to 90 s or 60 s respectively. Results: During the period of 60 s to 90 s, the median proportion of platform crossing times number was 50.0%(0.0%, 75.0%) in the AD group, which was significantly higher than that in the LM group 16.7 %(0.0%, 28.6 %)(P<0.05);during the period of 60 s to 90 s, the median proportion of target quadrant residence time was 45.2%(37.6 %, 52.8 %) in the AD group,which was significantly higher than that in the LM group 31.7 %(28.7 %, 40.9 %)(P<0.05). The AD group mice could obtain more reliable objective experimental information in the incubation period of 90 s compared with 60 s. Conclusion: When Morris water maze was used to detect the cognitive dysfunction of AD transgenic mice, setting the escape latency threshold to 90 s rather than 60 s could reflect the impairment of spatial learning and memory ability better.
作者
张莹林
郭依清
张童
蒋明
沈阳
姚俊岩
ZHANG Ying-lin;GUO Yi-qing;ZHANG Tong;JIANG Ming;SHEN Yang;YAO Jun-yan(Department of Anesthesiology,Shanghai Jiao Tong University School of Medicine,Shanghai general Hospital,Shanghai Jiaotong University,Shanghai,200080,China;Department of Anesthesiology,Zhongshan Hospital,Fudan University,Shanghai,200030,China)
出处
《现代生物医学进展》
CAS
2020年第8期1431-1435,1440,共6页
Progress in Modern Biomedicine
基金
国家自然科学基金面上项目(81771269)
上海市浦江人才计划资助项目(17PJD035)。
