摘要
为了考察凡纳滨对虾蛋白的体外降脂作用,以胆酸盐吸附能力、胰脂肪酶抑制率、降胆固醇作用、胆固醇酯酶反应作用等为研究指标,对凡纳滨对虾蛋白不同pH沉淀组分降脂特征进行研究。结果表明,凡纳滨对虾蛋白含量为18.40%,不同分离组分蛋白对胆酸盐有很好的吸附能力,其中对牛磺胆酸钠吸附能力最强的是pH6组分(91.3%),对甘氨胆酸钠吸附能力最强的是pH6组分(75.1%),对脱氧胆酸钠吸附能力最强的是pH4组分(63%);pH7组分对胰脂肪酶的抑制率高达76.8%;降胆固醇作用最高是pH9组分(83.9%),最低为pH7组分(67.3%);而不同组分对胆固醇酯酶的抑制率存在较大差异,最高为pH10组分(83%),最低为pH4组分(23%)。综上,凡纳滨对虾蛋白有一定的体外降脂功效。
To investigate the hypolipidemic effect of Penaeus vannamei protein in vitro,the hypolipidemic characteristics of different precipitated components of Penaeus vannamei protein were studied by cholate adsorption capacity,inhibition rate of pancreatic lipase,hypolipidemic effect and cholesterol esterase reaction.The results showed that the protein content of Penaeus vannamei was 18.40%,and the proteins from different parts had very good adsorbability for cholate.The strongest adsorption capacity part was pH6 part for sodium taurocholate(91.3%),while pH6 part for sodium glycochlorate(75.1%),and pH4 for sodium deoxycholate(63%).The pH7 part had the highest inhibition rate of pancreatic lipase(76.8%).The highest part of cholesterol-lowering effect was pH9(83.9%)and the lowest part was pH7(67.3%).The inhibition rates of cholesterol esterase were different,the highest and the lowest are pH10(83%)and pH4(23%),respectively.The proteins from Penaeus vannamei have a certain effect on reducing cholesterol in vitro.
作者
龚受基
蒙彦妃
曹惠怡
刘忠义
GONG Shou-ji;MENG Yan-fei;CAO Hui-yi;LIU Zhong-yi(College of Food Engineering,Beibu Gulf University,Qinzhou 535011,China)
出处
《食品工业科技》
CAS
北大核心
2020年第11期316-321,共6页
Science and Technology of Food Industry
基金
北部湾大学校级科研项目(2017KYQD223)
广西高校科学技术研究项目(KY2015ZD086)。
关键词
凡纳滨对虾
蛋白质
降脂
Penaeus vannamei
protein
hypolipidemic effect
