摘要
研究显示,鲍曼不动杆菌的院内持续传播与反复发作与生物膜形成密切相关。生物膜的形成受群体行为模式调控,研究发现通过添加自诱导剂类似物,可以竞争性抑制细菌自诱导体的作用从而抑制生物膜的生成。以邻氨基苯甲酰胺为母体结构,合成出20个化合物,并测试其对鲍曼不动杆菌生物膜的抑制活性。研究发现在50μmol/L的抑制浓度下,大部分化合物均显示出对鲍曼不动杆菌一定的生物膜抑制活性,其中化合物N,N-二苄基-2-苄基氨基苯甲酰胺、2-苄基氨基-N-氨基甲酰胺、2-苄基氨基-N-(4-羟基苯基)苯甲酰胺在50μmol/L条件下生物膜抑制率超过30%,通过细菌MIC试验,证明了化合物对细菌无杀灭作用而是抑制了细菌生物膜。
The work has shown that the persistent transmission and recurrence of Acinetobacter baumannii infection in hospital is closely related to biofilm formation.The formation of biofilm is controlled by quorum sensing.The formation of biofilm can be inhibited by adding auto-inducer analogues,which can competitively inhibit the effect auto-inducer.20 Compounds were synthesized using o-aminobenzamide as the parent structure,and their inhibitory activities on Acinetobacter baumannii biofilm were tested.The most of the compounds exhibited certain biofilm inhibitory activity at the inhibitory concentration of 50μmol/L.Among them,compounds N,N-dibenzyl-2-(benzylamino)benzamide,2-(benzylamino)-N-(diaminomethylene)benzamide and 2-(benzylamino)-N-(4-hydroxyphenyl)benzamide exhibited more than 30%biofilm inhibitory activity at the inhibitory concentration of 50μmol/L.The inhibitory effect of the synthetic compounds on biofilm was further illustrated by the MIC test,and the results shows that the compounds inhibit the biofilm formation instead of kill the bacteria.
作者
薛维娜
杨果
程铖
李应贤
杨元勇
XUE Wei-na;YANG Guo;CHENG Cheng;LI Ying-xian;YANG Yuan-yong(School of Medicine and Health Management,Guizhou Medical University,Guiyang 550025,China;School of Pharmacy,Guizhou Medical University,Guiyang 550025,China;School of Medicine and Pharmacy,Ocean University of China,Qingdao 266100,China)
出处
《化学试剂》
CAS
北大核心
2020年第5期493-499,共7页
Chemical Reagents
基金
国家自然科学基金资助项目(81660348)
贵阳市科技局基金项目([20151001]06)
黔科合平台人才项目([2018]5779-62)
贵阳市卫计委科学技术基金项目(gzwjkj2018-1-059)。
