摘要
目的:筛选口腔鳞状细胞癌(OSCC)患者外周血差异表达的microRNA(miRNA),探讨其与临床参数及其预后意义。方法:利用qRT-PCR技术检测OSCC外周血中miRNA的变化,扩大样本量进行验证,并分析miRNA与OSCC临床病理指标和预后的关系。结果:总共检测84种miRNA,其中OSCC患者外周血中上调大于2倍的miRNA有8个;下调小于0.5倍的有18个。其中miR-222-3p和miR-423-5p在外周血的表达与pTNM分期和淋巴结转移显著相关(P<0.05)。miR-222-3p高表达和miR-423-5p低表达的患者生存期均较短(P<0.05)。多因素分析结果提示miR-222-3p高表达可作为OSCC患者生存的独立预后因素。结论:miR-222-3p和miR-423-5p在OSCC患者外周血中存在差异表达,并有望成为OSCC早期诊断和预测预后的生物标志物。
Objective:To screen differentially expressed microRNAs(miRNAs)in peripheral blood of patients with oral squamous cell carcinoma(OSCC)by qRT-PCR,and further to explore the relationship between differentially expressed miRNAs and clinical parameters and prognosis.Methods:The changes of miRNA in peripheral blood of patients with OSCC were observed by qRT-PCR,and the relationship between miRNA and clinicopathological parameters and prognosis was analyzed.Results:A total of 84 kinds of miRNAs were detected,of which 8 were up-regulated more than twice in peripheral blood of patients with OSCC and 18 were down-regulated less than 0.5 times.The expression of miR-222-3p and miR-423-5p in peripheral blood was significantly correlated with pTNM stage and lymph node metastasis(P<0.05).The patients with high expression of miR-222-3p and low expression of miR-423-5p had a worse prognosis(P<0.05).Multivariate analysis showed that the overexpression of miR-222-3p was an independent prognostic factor for the OSCC patients.Conclusions:miR-222-3p and miR-423-5p were abnormally expressed in the peripheral blood in OSCC patients,and they were expected to be biomarkers for early diagnosis and predicting prognosis of OSCC.
作者
杨凯成
杨蕾
赵建广
罗风玉
陈彦平
崔子峰
陈赫
满莎莎
YANG Kaicheng;YANG Lei;ZHAO Jianguang;LUO Fengyu;CHEN Yanping;CUI Zifeng;CHEN He;MAN Shasha(Department of Stomatology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China;Department of Stomatology, People's Hospital of Lingshou County, Shijiazhuang 050500, China;Department of Stomatology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, China)
出处
《口腔医学研究》
CAS
北大核心
2020年第5期428-432,共5页
Journal of Oral Science Research
基金
河北省卫生厅青年科技课题(编号:20190728)。
