摘要
从罗源湾红树林根际土壤中分离解磷菌,筛选出洋葱伯克霍尔德菌(Burkholderia cepacia,NR113645.1)和短小芽孢杆菌(Bacillus pumilus,NR043242.1),研究它们的解磷特性和动态解磷过程.HPLC结果表明,B.cepacia菌液上清中含葡萄糖酸、丙酮酸、乳酸、乙酸、丁二酸,这些有机酸的产生导致磷酸盐溶解.B.pumilus菌液上清中仅检测到少量的葡萄糖酸,因此该菌溶磷效果不佳.在细菌动态溶磷过程中发现,经B.cepacia菌处理的磷酸钙,其X射线衍射(XRD)特征峰强度随时间的增长降低,显微镜图像显示磷酸钙颗粒随时间增长变小,甚至消失.而经B.pumilus菌处理的结果表明,在试验期间内磷酸钙峰强度和颗粒大小没有明显变化.细菌产生有机酸是溶解磷酸盐的重要前提,这是解决板结土壤中难溶性磷源转化为生物可利用磷源问题的关键.
The strains of Burkholderia cepacia(NR 113645.1)and Bacillus pumilus(NR 043242.1)were isolated from the mangrove rhizosphere soil of Luoyuan Bay.Their phosphate-decomposing characteristics and dynamic phosphorus-decomposing process were characterized.HPLC results showed that the supernatant of B.cepacia bacteria contained gluconic acid,pyruvate,lactic acid,acetic acid,and succinic acid.The production of these organic acids led to the dissolution of phosphate,resulting in that very little gluconic acid in supernatant and a bad phosphate-dissolving effect of B.pumilus.During the dynamic phosphorus dissolution of B.cepacia,The X-ray diffraction(XRD)characteristic peak intensity of calcium phosphate was dropped and the calcium phosphate particles became smaller or even disappeared with the prolonged treatment time.In contrast,XRD and microscope results showed there were no significant changes in the calcium phosphate's characteristic peak intensity and particle size when the same experiment was performed in B.pumilus.Dissolving phosphate is the key to solving the problem of converting insoluble phosphorus source into bioavailable phosphorus source in compacted soil.
作者
骆韵涵
柯志滨
钟超
程扬健
LUO Yun-han;KE Zhi-bin;ZHONG Chao;CHENG Yang-jian(College of Environment and Resources,Fuzhou University,Fuzhou 350108,China;Institute of Advanced Manufacturing,Fuzhou University,Jinjiang 362251,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2020年第6期2664-2673,共10页
China Environmental Science
基金
国家重点基础研究发展(973)计划项目(2014CB846003)
福建省自然科学基金项目(2019J01246)
泉州市科技计划项目(2019N118S)。
关键词
红树林
解磷菌
有机酸
生物可利用磷
动态解磷特性
mangrove
phosphate-solubilizing bacteria
organic acids
bioavailable phosphorus
real-time phosphate-solubilizing characteristics
