摘要
为探究油菜(Brasscia napu L.)氮素吸收和根系性状对低氮胁迫的响应,选用常规油菜种植品种"814"和湘油15为材料,采用水培,以正常供氮(NO3-浓度为10 mmol/L)为对照(CK),设定2个不同低氮水平(NO3-浓度分别为3、1 mmol/L)处理,测定油菜在不同氮素水平下的氮素吸收量及氮素吸收效率,探究油菜根冠比、根体积、根系吸收面积、根系直径、总根长和根系分枝数的变化特征及其与氮素吸收效率的相关性,并测定了油菜根系活力和根系质膜H+-ATP酶活性变化。结果表明,与CK相比,油菜在低氮胁迫下氮素吸收总量降低,但氮素吸收效率提高,油菜根系细长化且分枝增多,根体积下降但根系吸收面积上升。根系形态和氮素吸收效率的相关性分析表明,影响油菜氮素吸收效率的最大正相关和负相关因素是总根长和根系直径。油菜根系活力CK与3 mmol/L NO3-处理差异不显著,但极显著高于1 mmol/L NO3-处理。在低氮胁迫下,根系质膜H+-ATP酶活性显著升高,3个氮素水平下的油菜根系质膜H+-ATP酶活性差异均达显著水平。
In order to explore the response of nitrogen(N) uptake and root traits of rapeseed(Brasscia napus L.) to the low N stress, two conventional rapeseed varieties, "814" and Xiangyou15, with hydroponics cultured, were used as experimental material, and three nitrogen treatments 10, 3 and 1 mmol/L NO3- were set up with 10 mmol/L as control. The amount of N absorbed and its N-uptake efficiency of repseed were determined as well as the root-shoot ratio, root volume,root absorbing area, root diameter, the whole root length and the branching number were assayed. The correlation between the N-uptake efficiency and root morphological characters were analyzed. Moreover, the root activity and the activity of H+-ATPase in root plasma membrane were measured. The results showed that the amount of N absorbed by rapeseed was decreased, but the N-uptake efficiency increased under low-N stress. The roots of rapeseed were elongated and showed more branches with lower root volume and higher root absorbing area in the low-N treatments compared with the control. The root activities showed no significant difference between the treatment with control and the treatment with 3 mmol/L NO3- supplied, however, the root activity showed significantly decreased with 1 mmol/L NO3- supplied. Compared with normal N supplied, the activities of H+-ATPase in root plasma membrane of rapeseed were significantly increased under low N stress. It showed significant difference on the H+-ATPase activity of rapeseed among the treatments with 10 mmol/L, 3 mmol/L and 1 mmol/L N supplied.
作者
陈卓
胡怡凡
韩永亮
CHEN Zhuo;HU Yi-fan;HAN Yong-liang(College of Resources and Environment,Hunan Agricultural University,Changsha 410128,China)
出处
《湖北农业科学》
2020年第5期57-62,共6页
Hubei Agricultural Sciences
基金
湖南省教育厅科学研究项目(17K042)
土壤与农业可持续发展国家重点实验室开放课题(Y20160015)。
