摘要
以离子液体为提取剂,采用离子液体-超声波辅助提取黑豆异黄酮,考察了离子液体种类、浓度、料液比、时间以及超声功率对黑豆异黄酮提取量的影响,采用响应曲面法对提取工艺进行优化。结果表明,黑豆异黄酮的最佳提取条件为:选择[Bmim]Br为提取溶剂,离子液体浓度0.57 mol/L,料液比1:21,提取时间40 min,超声功率350 W。在此条件下,黑豆异黄酮的提取量为6.11 mg/g,预测值和实际测定值接近。该方法提取时间短、提取效率高,可为黑豆中天然异黄酮的开发利用提供了科学数据。
The isoflavone was extracted from black soybean by using ionic liquid ultrasonic assisted method with ionic liquid as extractant.The influence of ionic liquids species,ionic liquids concentration,liquid-solid ratio,ultrasonic time,ultrasonic wave power on yield were investigated.The extraction parameters were optimized by the response surface methodology.At the same time,the antibacterial effect of isoflavones from black soybean was researched in vitro.The optimum conditions were shown as follows:[Bmim]Br concentration was 0.57 mol/L,liquid-solid ratio was 121(g/mL),ultrasonic time was 40 min,ultrasonic wave power was 350 W.Under the above conditions,the isoflavones yield of the black soybean was 6.11 mg/g,the predicted value was close to the actual measured value,and extraction rate was higher than traditional extraction method.Box-behnken design combined with response surface analysis can optimize the extraction conditions of isoflavones in black soybean.By using ionic liquids based ultrasonic assisted extraction method,isoflavones were extracted from black soybean stably and feasibly.This study could provide scientific data for the application of black soybean as a natural isoflavones source.
作者
黎莉
于德涵
苏适
王广慧
LI Li;YU De-han;SU Shi;WANG Guang-hui(Food and Pharmaceutical Engineering Department,Suihua University,Heilongjiang Suihua 152061,China)
出处
《广州化工》
CAS
2020年第12期73-76,共4页
GuangZhou Chemical Industry
基金
黑龙江省基本科研业务费项目(KYYWF10236180105)。
关键词
黑豆
异黄酮
离子液体
超声辅助提取
black soybean
isoflavone
ionic liquid
ultrasonic-assisted method
