摘要
调查发现河南省开封市祥符区和郑州市中牟县西瓜田病毒病严重发生,症状表现为叶片黄化、蕨叶、皱缩、卷曲,枝梢翘起,果实变小和瓜纹不清。为明确其病毒种类,通过小RNA测序及生物信息学分析发现采集的西瓜样本中存在8种病毒。其中包括5种已知病毒:小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)、甜瓜蚜传黄化病毒(Melon aphid-borne yellows virus,MABYV)、西瓜花叶病毒(Watermelon mosaic virus,WMV)、黄瓜绿斑驳花叶病毒(Cucumber green mottle mo-saic virus,CGMMV)和西瓜隐潜病毒(Citrullus lanatus cryptic virus,CiLCV);3种为新检出的RNA病毒:西瓜病毒A(Watermelon virus A,WVA)、西瓜皱叶相关病毒1号(Watermelon crinkle leaf-associated virus 1,WCLaV-1)和西瓜皱叶相关病毒2号(Watermelon crinkle leaf-associated virus 2,WCLaV-2)。RT-PCR检测田间66个西瓜标样发现,WMV、CiLCV和ZYMV是侵染两地西瓜的主要病毒,其阳性检出率分别为61%、56%和52%,两种及以上病毒复合侵染的比率达68%。
During 2015 and 2016,severe viral disease occurred in watermelon fields in Kaifeng and Zhongmu,Henan Province.Complex symptoms include yellowing,fern leaves,shrinking,curling,topmost upwarping,fruit shrunking and fuzziness of fruit pattern,which lead to difficulty for identifying pathogens.In this research,we combined small RNA sequencing and bioinformatic analysis to reveal the viruses in collected symptomatic watermelon samples.Ultimately,eight viruses were discovered including Zucchini yellow mosaic virus(ZYMV),Melon aphid-borne yellows virus(MABYV),Watermelon mosaic virus(WMV),Cucumber green mottle mosaic virus(CGMMV)and Citrullus lanatus cryptic virus(CiLCV)which have been reported in China.Other three new viruses,Watermelon virus A(WVA),Watermelon crinkle leaf-associated virus 1(WCLaV-1)and Watermelon crinkle leaf-associated virus 2(WCLaV-2)reported by our laboratory in recent years,were also detected in the samples.After testing of 66 samples collected from different fields by RT-PCR,we found WMV,CiLCV and ZYMV as the three most common viruses in samples,with frequency 61%,56%and 52%,respectively.Meanwhile,we also found most samples infected by the complex viruses with 68%of infection rate.
作者
姜军
吴楠
辛敏
曹孟籍
王锡锋
JIANG Jun;WU Nan;XIN Min;CAO Meng-ji;WANG Xi-feng(Xiangfu Institute of Agricultural Sciences,Kaifeng 475100,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;National Citrus Engineering Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2020年第3期286-291,共6页
Acta Phytopathologica Sinica
基金
中国农业科学院科技创新工程(ASTIP02-02)。
关键词
西瓜
小RNA测序
病毒病
复合侵染
watermelon
small RNA
viral disease
complex infection
