摘要
目的探讨miRNA-193b、Lp-PLA2、miR-146a与阿尔茨海默病(AD)严重程度的关系及预测疗效的价值。方法选取2016年1月至2019年6月本院收治的114例AD患者,根据病情分为轻度组(n=35)、中度组(n=49)、重度组(n=30),比较各组miRNA-193b mRNA、Lp-PLA2、miR-146a m RNA水平、简易精神状态检查量表(MMSE)、神经精神问卷(NPI)评分,采用Spearman分析各指标与病情分级的相关性,采用Pearson分析各指标与MMSE、NPI评分的相关性,采用接收者操作特征(ROC)曲线及ROC下面积(AUC)分析各指标预测疗效的价值。结果 miRNA-193b mRNA与病情分级呈负相关,与MMSE评分呈正相关;与NPI评分呈负相关;Lp-PLA2、miR-146a m RNA与病情分级呈正相关,与MMSE评分呈负相关,与NPI评分呈正相关,差异有统计学意义(P<0.05);miRNA-193b m RNA与Lp-PLA2、miR-146a mRNA呈负相关,Lp-PLA2与miR-146a m RNA呈正相关(P<0.05);miR-146a mRNA预测疗效的AUC为0.796,高于miRNA-193b mRNA预测疗效AUC的0.766及Lp-PLA2预测疗效AUC的0.741,但低于3指标联合的AUC(P<0.05)。结论 miRNA-193b、Lp-PLA2、miR-146a与AD严重程度显著相关,3者可相互影响,共同参与AD病情进展,检测3者表达有望成为预测疗效的新型方案。
Objective To investigate the relationship between miRNA-193b,Lp-PLA2,miR-146a and the severity of Alzheimer's disease(AD)and the value of predicting efficacy.Methods From January 2016 to June 2019,114 patients with AD who were treated in our hospital were selected,and divided into mild group(n=35),moderate group(n=49)and severe group(n=30)according to the condition,the levels of miRNA-193b mRNA,Lp-PLA2,miR-146a mRNA,Mini Mental State Examination Scale(MMSE),and Neuropsychiatric Questionnaire(NPI)scores in each group were compared,Spearman was used to analyze the correlation between each index and disease grade,and Pearson was used to analyze the correlation between each index and MMSE and NPI scores,the receiver operating characteristic(ROC)curve and the area under the ROC(AUC)were used to analyze the predictive value of each index.Results miRNA-193b was negatively correlated with disease grade,positively correlated with MMSE score,and negatively correlated with NPI score.Lp-PLA2,miR-146a was positively correlated with the disease grade,negatively correlated with the MMSE score,and positively correlated with the NPI score(P<0.05).miRNA-193b was negatively correlated with Lp-PLA2 and miR-146a,Lp-PLA2 was positively correlated with miR-146a(P<0.05).The AUC of miR-146a predictive effect was 0.796,which was higher than that of miRNA-193b predictive effect AUC of 0.766 and Lp-PLA2 predictive effect AUC of 0.741,but lower than the combined AUC of the three indicators(P<0.05).Conclusion miRNA-193b,Lp-PLA2,and miR-146a are significantly related to the severity of AD.The three can interact with each other and participate in the progression of AD.The detection of the expression of the three is expected to become a new method for predicting the efficacy.
作者
尚晓峰
冉秀荣
李虹
SHANG Xiaofeng;RAN Xiurong;LI Hong(Department of general medicine,shangqiu first people's hospital,Shangqiu,Henan,China,476000)
出处
《分子诊断与治疗杂志》
2020年第6期813-817,共5页
Journal of Molecular Diagnostics and Therapy
基金
河南省医学科技攻关计划项目(2011030040)。
