摘要
目的探讨黄芪(AS)对D-半乳糖(D-gal)致雌性小鼠卵巢衰老的改善作用及其抗氧化机制。方法将60只ICR雌鼠随机分为空白对照(CON)组、D-gal组、D-gal+AS组,每组20只。每组各取6只小鼠行体外受精实验,计数并比较各组的二细胞数、四细胞数及囊胚数;收集各组其余小鼠卵巢组织和血清,采用酶联免疫吸附(ELISA)法检测其中超氧化物歧化酶(SOD)和丙二醛(MDA)含量;采用逆转录-聚合酶链式反应(RT-PCR)法检测囊胚SOD1和SOD2基因表达。结果小鼠体外受精实验中,D-gal+AS组各阶段胚胎数均高于D-gal组(P <0. 05);D-gal+AS组卵巢和血清中SOD含量均高于D-gal组(P <0. 05,P <0. 01),MDA含量均低于D-gal组(P <0. 05);囊胚中D-gal+AS组SOD1和SOD2基因表达均高于D-gal组(P <0. 05)。结论黄芪可通过增加血液及卵巢组织中抗氧化物SOD含量,降低生殖系统氧化应激反应,并通过调控卵巢抗氧化基因的表达,有效改善卵巢衰老小鼠的生殖功能,可为临床应用黄芪防治卵巢衰老提供参考。
Objective To study the effect and mechanism of astragalus(AS)on ovarian aging induced by D-galactose(D-gal)in female mice.Methods Sixty ICR females were randomly divided into the control group(CON)group,D-gal group and D-gal+AS group,20 rats in each group.Six mice in each group were subjected to in vitro fertilization experiments,comparing the number of two cells,four cells and blastocysts in each group.The ovarian tissues and serum of the other rats were collected,and the contents of SOD(superoxide dismutase)and MDA(malondialdehyde)were detected by enzyme-linked immuno sorbent assay(ELISA).SOD1 and SOD2 gene expressions in blastocysts were detected by reverse transcription-polymerase chain reaction(RT-PCR).Results The number of embryos in D-gal+AS group was higher than that in D-gal group(P<0.05).The content of SOD in D-gal+AS group was higher than that in D-gal group(P<0.05,P<0.01),and the MDA content was lower than that in D-gal group(P<0.05).SOD1 and SOD2 gene expressions in D-gal+AS group was higher than that in D-gal group(P<0.05).Conclusion Astragalus can effectively reduce the oxidative stress response of the reproductive system by increasing the content of antioxidant SOD and effectively improving the reproductive function of ovarian aging mice by regulating the expression of ovarian antioxidant genes.The results provide an important experimental theoretical basis for the clinical application of jaundice in the prevention and treatment of ovarian aging.
作者
朱嵩岳
伯乐
杨光照
尹娜
吴晨婷
茅彩萍
ZHU Songyue;BO Le;YANG Guangzhao;YIN Na;WU Chenting;MAO Caiping(Reproductive Medicine Center,The First Affiliated Hospital of Soochow University,Suzhou,Jiangsu,China 215006)
出处
《中国药业》
CAS
2020年第13期44-47,共4页
China Pharmaceuticals
基金
国家自然科学基金[81671535]
中华医学会临床医学科研专项资金-生殖医学青年医师研究与发展项目执行书[17020270696]。
关键词
黄芪
卵巢衰老模型
胚胎
抗氧化作用
astragalus
ovarian aging model
embryo
antioxidant effect
