摘要
以野生大叶石蝴蝶叶片为外植体,对影响大叶石蝴蝶植株再生的激素组合、接种方式、叶片大小、叶片切割方式等进行试验研究。结果表明,采用0.1%的升汞消毒12 min后,接种在MS+0.5 mg/L 6-BA+0.1 mg/L NAA+30 g/L蔗糖+5.0 g/L琼脂培养基上,培养120 d后可获得分化的无菌苗;无菌苗叶片转接在MS+1.0 mg/L 6-BA+0.1 mg/L NAA+30 g/L蔗糖+5.0 g/L琼脂上,50 d后的分化率为100%,平均每个叶片有效的不定芽数量为6个;其中以较大的叶片横切后,近轴面朝上接种在培养基上最有利于叶片分化;适宜生根的培养基为1/2MS+0.9 mg/L NAA+0.2 g/L AC+15 g/L蔗糖+6.0 g/L琼脂,生根率为100%。生根后移栽在腐殖土∶珍珠岩=5∶1基质上,注意控温控湿即可成活。
The leaves of wild Petrocosmea grandifolia were used as explants,and the hormone combination,inoculation method,leaf size and cutting methods that affected plant regeneration were studied in this experiment.The results showed that after disinfection with 0.1%mercury for 12 minutes,inoculated on MS+0.5 mg/L 6-BA+0.1 mg/L NAA+30 g/L sucrose+5.0 g/L agar and cultured for 120 days,the differentiated sterile seedlings could be obtained.The leaves of the seedling were transferred on MS+1.0 mg/L 6-BA+0.1 mg/L NAA+30 g/L sucrose+5.0 g/L agar,and after 50 days,the differentiation rate was 100%,the average number of available adventitious buds per leaf was 6.After crosscutting,the larger leaves were inoculated on the medium with the proximal axis facing upward,which was most conducive to the differentiation of leaves.The medium suitable for rooting was 1/2 MS+0.9 mg/L NAA+0.2 g/L AC+15 g/L sucrose+6.0 g/L agar,with rooting rate of 100%.After rooting,the plant could survive transplanting on humus soil∶perlite=5∶1 substrate,and paying attention to temperature and humidity.
作者
盛耀莹
郑晟靖
庭禹洁
金英
唐军荣
SHENG Yaoying;ZHENG Shengjing;TING Yujie;JIN Ying;TANG Junrong(Honghe Station of Forestry,Mengzi,Yunnan 661100,China;Honghe Team of Forestry and Grassland Inventory and Planning,Mengzi,Yunnan 661100,China;Key Laboratory of State Forestry and Grassland Administration on Biodiversity Conservation in Southwest China,Southwest Forestry University,Kunming 650224,China)
出处
《林业调查规划》
2020年第4期23-28,60,共7页
Forest Inventory and Planning
基金
云南省高校林木遗传改良与繁育重点实验室开放基金资助项目(YNGBT 201705).
关键词
大叶石蝴蝶
再生体系
叶片分化
激素配比
不定芽
生根率
移栽炼苗
Petrocosmea grandifolia
regeneration system
leaf differentiation
hormone ratio
adventitious bud
rooting rate
seedling transplanting and acclimatization
