摘要
目的观察摩腹法干预前后广泛性焦虑症(GAD)大鼠模型情感环路各核团组织的结构与功能变化,探讨摩腹法干预GAD的中枢调节机制。方法将60只Wistar雄性大鼠按随机数字表法分为空白组、模型组和摩腹组,每组20只。模型组和摩腹组均采用慢性情绪应激法建立GAD大鼠模型,并以高架十字迷宫实验对其进行评价。摩腹组大鼠建立GAD模型后给予摩腹干预,每天治疗1次,每次10 min,连续治疗14 d;模型组大鼠建立GAD模型后每天被束缚于实验台10 min,不予治疗,连续14 d;空白组大鼠不予任何干预。采用辣根过氧化物酶(HRP)逆行追踪标记技术检测各组大鼠情感环路核团组织的结构变化,采用液相色谱-质谱联用技术检测各组大鼠情感环路核团组织神经元代谢产物N-乙酰天冬氨酸(NAA)、谷氨酸、胆碱复合物(Cho)和肌酸的相对浓度变化。结果与空白组相比,模型组大鼠情感环路中HRP标记细胞分布较少,情感环路活动较弱;模型组大鼠左侧海马组织中的NAA/肌酸比值明显升高(0.94±0.08比0.79±0.10,P<0.05),右侧海马组织中的谷氨酸/肌酸比值明显降低(0.95±0.10比1.12±0.13,P<0.01),左侧皮质组织中的NAA/肌酸和谷氨酸/肌酸比值均明显降低(1.04±0.05比1.41±0.23,1.21±0.04比1.57±0.11,均P<0.01)。与模型组相比,摩腹组大鼠海马组织中有大量HRP聚集并向四周组织扩散;摩腹组左侧海马组织中的NAA/肌酸与谷氨酸/肌酸比值明显降低(0.74±0.21比0.94±0.08,0.92±0.20比1.21±0.12,均P<0.01),右侧海马组织中的谷氨酸/肌酸比值(1.01±0.23比0.95±0.10)与左侧皮质组织中的NAA/肌酸比值(1.12±0.09比1.04±0.05)和谷氨酸/肌酸比值(1.22±0.12比1.21±0.04)均较模型组升高,差异均无统计学意义(均P>0.05)。结论摩腹法能有效增强情感环路活动,改善情感环路中海马组织与皮质组织神经元代谢产物的相对浓度,提示摩腹法治疗GAD的作用机制可能与其调节情感环路的结构与功能密切相关。
Objective To observe the structural and functional changes of each nucleus group in generalized anxiety disorder(GAD)rat models before and after the massage therapy,and to explore the central regulating mechanism of the massage therapy in GAD.Methods 60 Wistar male rats were randomly divided into blank group,model group and abdominal rubbing group,20 rats for each group.GAD rat models were established by chronic emotional stress,and the models were evaluated by the elevated cross maze experiment.In the abdominal rubbing group,the GAD rats were treated with abdominal rubbing once a day for 10 min for 14 days.In the control group,the GAD rats were only bound to the experimental platform once a day for 10 min for 14 days.Horseradish peroxidase(HRP)retrograde tracking marker method was used to detect the changes of the structure of each nuclear cluster in the affective loop of the GAD rats in each group.The liquid-mass spectrometry quantitative analysis was used to determine the relative concentrations of N-acetyl aspartate(NAA),glutamic acid,choline and creatine in nuclear cluster of the GAD rats in each group.Results Compared with the blank group,the HRP labeled cells in the rats of model group were less expressed and the activity of the emotional circuit in the brain was weak.The NAA/creatine ratio in the left hippocampal tissue of the rats in the model group was significantly increased than that of the blank group(0.94±0.08 vs 0.79±0.10,P<0.05),the glutamic acid/creatine ratio in the right hippocampal tissue was significantly decreased(0.95±0.10 vs 1.12±0.13,P<0.01),and the NAA/creatine andglutamic and acid/creatineratios in the left cortical tissue were all significantly decreased(1.04±0.05 vs 1.41±0.23,and 1.21±0.04 vs 1.57±0.11,all P<0.01).Compared with the model group,the hippocampal tissues of the GAD rats in the abdominal rubbing group had a large number of HRP aggregation and spread to the surrounding tissues,the NAA/creatine and glutamic acid/creatine ratios in the left hippocampal tissue of the abdominal rubbing group were significantly decreased(0.74±0.21 vs 0.94±0.09 and 0.92±0.20 vs 1.21±0.12,all P<0.01).The differences between the model group and the abdominal rubbing group in the ratio of glutamic acid/creatine in the right hippocampal tissue(1.01±0.23 vs 0.95±0.10),the ratio of NAA/creatine in the left cortical tissue(1.12±0.09 vs 1.04±0.05),and the ratio of glutamic acid/creatine in the left cortical tissue(1.22±0.12 vs 1.21±0.04)were not statistically significant(P>0.05).Conclusions Abdominal rubbing can effectively enhance the activity of affective loop and improve the relative concentration of neuron metabolites in hippocampal and cortical tissues in the affective loop,suggesting that the mechanism of GAD treatment by abdominal rubbing may be closely related to the structure and function of regulating affective loop.
作者
骆雄飞
刘斯文
李华南
张玮
王强松
孙庆
Luo Xiongfei;Liu Siwen;Li Huanan;Zhang Wei;Wang Qiangsong;Sun Qing(Department of Tuina,The First Teaching Hospital of Tianjin University of TCM,Laboratory for Biological Effects of Massage,State Administration of Traditional Chinese Medicine,Tianjin 300193,China;Institute of Biomedical Engineering,Chinese Academy of Medical Science&Peking Union Medical College,Tianjin 300192,China)
出处
《国际生物医学工程杂志》
CAS
2020年第1期11-17,共7页
International Journal of Biomedical Engineering
基金
国家自然科学基金面上项目(81674093,81473793)。
关键词
摩腹法
广泛性焦虑症
情感环路
中枢调节
Abdominal rubbing
Generalized anxiety disorder
Emotionalloop
Central adjustment
