摘要
【目的】通过监测2018—2019年度上海市优势流行株A/H1N1(pdm09)亚型流感病毒对神经氨酸酶抑制剂(NAIs)的耐药情况,为上海市流感临床用药提供参考。【方法】随机选取60株A/H1N1(pdm09)亚型流行株,采用神经氨酸酶抑制实验及神经氨酸酶(NA)基因测序的方法,开展流行株对奥司他韦及扎那米韦敏感性和耐药性的监测和分析。【结果】60株流行株均对奥司他韦和扎那米韦敏感,未发现敏感性降低或者显著降低的情况;基因序列分析也未发现NA在催化活性关键位点及辅助位点出现氨基酸变异,佐证了神经氨酸酶抑制实验的表型检测结果。【结论】上海市2018—2019年度流行的A/H1N1(pdm09)亚型流感病毒对神经氨酸酶抑制剂依然敏感,为临床用药提供了科学参考。随着药物的广泛使用,应持续加强流感病毒耐药性监测,以有效指导临床用药。
[Objective]A/H1N1(pdm09)viruses were the dominant strains in Shanghai during 2018-2019 influenza surveillance year.This study is to provide a scientific reference for clinical drug use by investigating the susceptibility of A/H1N1(pdm09)viruses to neuraminidase inhibitors(NAIs).[Methods]Sixty strains of A/H1N1(pdm09)viruses were randomly selected for testing the susceptibility and drug resistance to Oseltamivir and Zanamivir by means of neutaminidase inhibition and neuraminidase(NA)gene sequencing.[Results]The 60 epidemic strains all proved to be susceptible to Oseltamivir and Zanamivir and the susceptibility was not observed to be decreased or remarkably decreased.In genetic sequencing,NA was not observed to present amino acid mutation at the key sites and auxiliary sites in catalytic activity,which confirmed the results of the phototypic detection of neuraminidase inhibition.[Conclusion]The subtype influenza viruses A/H1N1(pdm09)circulating in Shanghai during 2018-2019 surveillance year are still sensitive to NAIs,which provides a scientific reference for clinical use of drugs.However,we monitored only a number of strains and think that the work monitoring antiviral susceptibility should be continued with the wide use of the drugs.
作者
赵雪
滕峥
房方皓
蒋慧
牟嘉斌
吴佳谨
姜晨彦
袁政安
林庆能
张曦
ZHAO Xue;TENG Zheng;FANG Fang-hao;JIANG Hui;MOU Jia-bin;WU Jia-jin;JIANG Chen-yan;YUAN Zheng-an;LIN Qing-neng;ZHANG Xi(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;Shanghai Songjiang District Center for Disease Control and Prevention,Shanghai 201600,China)
出处
《上海预防医学》
CAS
2020年第5期401-407,共7页
Shanghai Journal of Preventive Medicine
基金
“十三五”重大传染病防治科技重大专项(2017ZX10103009-003)
上海市自然科学基金(17ZR1424200)
上海市卫健委面上项目(20174Y0087)
上海市卫健委青年项目(2016Y0107)。
关键词
流感病毒
耐药性
神经氨酸酶
神经氨酸酶抑制剂
influenza virus
drug resistance
neuraminidase
neuraminidase inhibitors(NAIs)
