摘要
为了解采用改良Hoagland溶液进行水培炼苗对香蕉组培苗生长和光合特性的影响,以‘巴西蕉’组培苗为材料,进行直接移栽(G1)、正常炼苗移栽(G2)和水培一周后移栽(G3)处理,比较3组香蕉苗形态、生理特性和生长相关指标的差异.结果显示,与G1和G2组相比,G3组香蕉幼苗根长、地上部和地下部干重、根冠比极显著增加(P<0.01),根数显著增多(P<0.05).移栽后4个月,G3组株高、叶宽、叶长极显著大于G2组(P<0.01),大于G1组,但差异不显著.植株叶片叶绿素含量和根系活力由高到低均为G3组、G2组、G1组,且G3组极显著高于其他两组(P<0.01).移栽后4个月,G3组叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量与G1组相比分别增加49.0%、50.1%、49.4%和58.5%,与G2组相比分别增加23.98%、28.38%、25.43%和26.77%;G3组根系活力分别约为G1和G2组的3.04倍和1.86倍;G3组植株净光合速率、蒸腾速率、气孔导度、实际光合效率(YII)、电子传递速率(ETR)、光化学猝灭系数(q_P)也均极显著高于其他两组(P<0.01).本研究表明水培炼苗可以提高香蕉苗根系活力,加快植株生长,提高叶片叶绿素含量和光合效率,增强植株生物量的积累,说明水培炼苗具有应用于香蕉种苗繁育的巨大潜力.
The aim of this study is to investigate the effects of hydroponic culture hardening using modified Hoagland solution on the growth and photosynthetic characteristics of tissue cultured banana seedlings.Micro-propagated‘Brazil’banana seedlings were divided into three groups:direct transplanting(G1),transplanting after normal hardening(G2),and one week of hydroponic culture before transplanting(G3).Following transplantation,the morphological and physiological characteristics and growth related indexes of banana seedlings from these three groups were compared.Results showed that some growth and development indexes of G3 seedlings,including root length,aboveground and underground dry weight,and root-shoot ratio were significantly higher than those of G1 and G2(P<0.01).Furthermore,the root number of G3 seedlings was significantly greater than that of the other two groups(P<0.05).Four months after transplanting,the plant height,leaf width,and leaf length of G3 plants were significantly higher than those of G2(P<0.01).They were also higher than those of G1,although not significantly.The leaf chlorophyll content and root activity indexes were highest in G3,followed by G2,then G1,with these two indexes being significantly higher in G3 than in the other two groups(P<0.01).At four months post-transplantation,the chlorophyll a,chlorophyll b,total chlorophyll,and carotenoid contents in G3 leaves were 49.0%,50.1%,49.4%,and 58.5%higher,respectively,than G1 leaf contents.Similarly,they were 23.98%,28.38%,25.43%,and 26.77%higher,respectively,than contents of G2 leaves.The root activity of G3 plants was approximately 3.04 times and 1.86 times that of G1 and G2,respectively.Additionally,the net photosynthetic rate,transpiration rate,stomatal conductance,actual photosynthetic rate(YII),electron transport rate(ETR),and photochemical quenching coefficient(qP)of G3 seedlings were all found to be significantly higher than in the other two groups(P<0.01).Our results show that hydroponic culture prior to transplantation can increase root activity,enhance plant growth,and increase leaf chlorophyll content,and significantly promote plant photosynthetic efficiency and biomass accumulation in banana seedlings.This indicates that hydroponic culture hardening has great potential to be used in banana seedling propagation.
作者
田娜
刘范
孙雪丽
车婧如
项蕾蕾
赖钟雄
程春振
TIAN Na;LIU Fan;SUN Xueli;CHE Jingru;XIANG Leilei;LAI Zhongxiong;CHENG Chunzhen(College of Horticulture/Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第3期582-589,共8页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31601713)
福建农林大学“校杰出青年科研人才”计划项目(xjq201721)
福建农林大学科技创新专项基金项目(CXZX2018082)
国家现代农业产业技术体系专项资金(CARS-31-15)
福建省高原学科建设经费(102/71201801101)资助。
关键词
水培炼苗
香蕉
植株生长
根系活力
光合特性
hydroponic culture hardening
banana
plant growth
root activity
photosynthetic characteristics
