摘要
目的初步统计连云港地区20~79岁健康成人24 h尿蛋白水平的分布情况,并建立该指标的参考区间。方法采用完全随机化方法选取2019年6月至8月于本院体检中符合要求的志愿者257例,其中男120例、女137例,年龄20~79岁。运用AU5800全自动生化分析仪及其配套试剂检测24 h尿蛋白,并对数据的分布情况进行正态性检验,依据CLSI C28-A3和WS/T 402-2012《临床实验室检验项目参考区间的制定》指南建立24 h尿蛋白指标的参考区间。结果本地区健康成人24 h尿蛋白水平的分布呈非正态分布(W=0.916,P<0.05),且在性别、年龄分布上差异无统计学意义(性别:Z=0.44,Z*=3.10,P>0.05;年龄:Z=1.14,Z^*=3.10,P>0.05)。本研究应用邻苯三酚红钼比色法定量检测24 h尿蛋白建立的参考区间为<445 mg/24 h。结论24 h尿蛋白参考区间的建立可以充分发挥其临床价值,能够为各种肾脏疾病的诊治提供重要的参考依据。
Objective To statistically analyze 24-hour urinary protein level in healthy adults age 20-79 in Lianyungang,and to establish its reference interval.Methods 257 subjects age 20-79,including 120 males and 137 females,who met the requirements of physical examination in our hospital from June to August,2019 were enrolled by using a complete randomization method.The concentration of 24-hour urinary protein were detected by AU5800 automatic biochemical analyzer and its matching reagents.Normality test of data was also carried out.According to the guidelines of CLSI C28-A3 and WS/T 402-2012 from Clinical Laboratory Test Project Reference Interval Formulation,the reference interval of 24-hour urinary protein was established.Results The distribution of 24 h urinary protein level in the healthy adults was non-normal in this area(W=0.916,P<0.05),and there were no statistical differences in gender and age distribution(gender:Z=0.44,Z*=3.10,P>0.05;age:Z=1.14,Z^*=3.10,P>0.05).In this study,the reference interval for the quantitative detection of 24 h urinary protein by pyrogallol red molybdenum colorimetry was<445 mg/24 h.Conclusion The establishment of 24 h urinary protein reference interval can fully exert its clinical value and provide important references for the diagnosis and treatment of various kidney diseases.
作者
杨伏猛
张卫东
邱月
孙蓓
颜景栋
刘倩
Yang Fumeng;Zhang Weidong;Qiu Yue;Sun Bei;Yan Jingdong;Liu Qian(Department of Laboratory,Lianyungang Second People's Hospital,Kangda College,Nanjing Medical University,Lianyungang 222006,China)
出处
《国际医药卫生导报》
2020年第14期2007-2010,共4页
International Medicine and Health Guidance News
基金
南京医科大学康达学院科研发展基金重点项目(KD2019KYJJZD011)
连云港市卫生科研计划项目(201721)。
关键词
24h尿蛋白
邻苯三酚红钼比色法
参考区间
24-hour urinary protein
Pyrogallol red molybdenum colorimetry
Reference interval
