摘要
使用MiSeq高通量测序技术对15个奶豆腐样品中细菌多样性进行了解析。结果表明,新疆维吾尔自治区塔城地区奶豆腐中优势细菌门为Proteobacteria(变形菌门)、Frimicutes(硬壁菌门)、Actinobacteria(放线菌门)和Bacteroidetes(拟杆菌门)。在所有样品中均存在且相对含量大于1.0%的细菌属为Lactobacillus(乳杆菌属,19.66%)、Acetobacter(醋酸杆菌属,16.69%)、Lactococcus(乳球菌属,13.90%)、Enterobacter(肠杆菌属,11.42%)、Staphylococcus(葡萄球菌属,3.33%)、Acinetobacter(不动杆菌属,2.79%)和Streptococcus(链球菌属,1.14%)。核心分类操作单元矩阵OTU注释为乳酸杆菌属、Macrococcus(巨球菌属)、乳球菌属、肠杆菌属、不动杆菌属和醋酸杆菌属。由此可见,塔城地区奶豆腐中含有大量的细菌类群,且以乳杆菌属、醋酸杆菌属、乳球菌属和肠杆菌属细菌为主。
This study used MiSeq high-throughput sequencing technology to analyze the bacterial diversity in 15 dairy toufu samples.The results showed that the dominant bacteria in dairy toufu in Tacheng area of Xinjiang Uygur Autonomous Region were Proteobacteria,Frimicutes,Actinobacteria and Bacteroidetes.The bacterial genus presented in all samples and with the relative abundance more than 1.0%were Lactobacillus(19.66%),Acetobacter(16.69%),Lactococcus(13.90%),Enterobacter(11.42%),Staphylococcus(3.33%),Acinetobacter(2.79%)and Streptococcus(1.14%).The core operational taxonomic units were identified as Lactobacillus,Macrorococcus,Lactococcus,Enterobacter,Acinetobacter and Acetobacter.It can be seen that the dairy toufu in Tacheng area contains a large number of bacterial groups,and was mainly composed of Lactobacillus,Acetobacter,Lactococcus and Enterobacter.
作者
雷炎
周亚澳
单春会
蔡文超
宋凯
郭壮
LEI Yan;ZHOU Yaao;SHAN Chunhui;CAI Wenchao;SONG Kai;GUO Zhuang(College of Food Science and Technology,Hu Bei University of Arts and Science,Xiangyang 441053,China;Food College,Shihezi University,Shihezi 832003,China)
出处
《中国乳品工业》
CAS
北大核心
2020年第6期9-13,共5页
China Dairy Industry
基金
新疆生产建设兵团重点领域创新团队建设计划项目(2017CB012)
湖北省荆楚卓越工程师协同育人计划(201657)。
关键词
奶豆腐
高通量测序
细菌多样性
核心菌群
Dairy toufu
High-throughput sequencing
Bacterial diversity
Core microbiota
