摘要
目的:用10倍模拟体液(10×SBF)仿生矿化丝素/壳聚糖(SF/CS)电纺支架制备骨仿生支架,并对比矿化前后支架的物理性能及诱导细胞成骨分化的能力。方法:通过扫描电镜(SEM)、傅里叶红外光谱(FT-IR)、吸水率和力学性能检测对比矿化前后支架成分及物理性能。通过活细胞染色和Cell Counting Kit-8(CCK-8)对比仿生矿化对于人骨髓间充质干细胞(HBMSC)在支架上黏附和增殖的影响。通过碱性磷酸酶(ALP)染色和茜素红染色(ARS)对比支架矿化前后对HBMSC成骨分化的诱导能力。结果:SEM、FT-IR、吸水率和力学性能证实了矿化的成功及支架物理性能的提高。活细胞染色和CCK8证明仿生矿化行为并未增加支架的细胞毒性,ALP和ARS证明矿化后支架更易诱导HBMSC的成骨分化。结论:经SBF仿生矿化后支架较原支架更符合骨组织工程的要求。
Objective:This study aims to prepare bionic bone scaffolds using 10 times simulated body fluid(10×SBF)mineralized silk fibroin/chitosan(SF/CS)electrospun scaffolds,and to compare the physical properties and the ability of inducing osteogenic differentiation of cells before and after mineralization.Methods:The compositions and properties of the scaffolds before and after mineralization were compared by scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FT-IR),water absorption and mechanical properties.The effects of biomimetic mineralization on the adhesion and proliferation of human bone marrow mesenchymal stem cells(HBMSC)on scaffolds were compared by living cell staining and Cell Counting Kit-8(CCK-8).Alkaline phosphatase(ALP)staining and alizarin red staining(ARS)were used to compare the osteoinductive ability of the scaffolds before and after mineralization.Results:SEM,FT-IR,water absorption and mechanical properties confirmed the success of mineralization and the improvement of physical properties.Live cell staining and CCK-8 showed that biomimetic mineralization did not increase the cytotoxicity of the scaffolds.ALP and ARS showed that the scaffolds after mineralization were more likely to induce osteogenic differentiation of HBMSC.Conclusion:SBF biomimetic mineralized scaffolds are more suitable for bone tissue engineering.
作者
龚春玲
陈飞扬
卜寿山
庄海
GONG Chunling;CHEN Feiyang;BU Shoushan;ZHUANG Hai(Department of Stomatology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第5期748-753,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金项目(81670967)。
关键词
仿生矿化
模拟体液
骨仿生支架
biomimetic mineralization
simulated body fluids
bionic bone scaffold
