摘要
目的探讨血管内皮生长因子(VEGF)、基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)表达与肝癌患者临床病理特征及预后的关系,为肝癌发生发展机制提供依据。方法选择2015年4月至2016年3月在义乌市中心医院手术切除的肝癌组织95例,选择因肝外伤切除的正常肝组织标本45例作为对照。采用免疫组化染色法检测肝癌组织及正常肝组织VEGF、MMP-2、MMP-9表达情况,并分析VEGF、MMP-2、MMP-9表达与患者临床病理及预后的相关性。结果肝癌组织中VEGF、MMP-2以及MMP-9阳性表达率均明显高于正常肝组织(81.05%、75.79%、84.21%比42.22%、33.33%、48.89%)(χ^2=21.364、23.397、19.265,均P<0.05)。肝癌组织低分化、TNM分期Ⅲ~Ⅳ期以及有血管侵袭患者肝癌组织VEGF、MMP-2、MMP-9表达阳性率均明显升高(均P<0.05)。肝癌组织VEGF、MMP-2以及MMP-9阴性表达患者生存情况均显著优于阳性表达患者(logχ^2=3.271、2.975、3.065,均P<0.05)。结论肝癌组织中VEGF、MMP-2以及MMP-9高表达,在肿瘤生长浸润和转移过程中发挥了重要作用,因此VEGF、MMP-2以及MMP-9可作为肝癌进展的生物学指标。
Objective To investigate the correlation between the expression of vascular endothelial growth factor(VEGF),MMP-2,MMP-9 and the clinicopathology and prognosis of patients with hepatocellular carcinoma(HCC),and to provide evidence for the occurrence and development of HCC.Methods From April 2015 to March 2016,95 HCC tissues were resected in Yiwu Central Hospital.Forty-fivenormal liver tissues which were resected due to liver trauma were selected as control group.Immunohistochemical staining was used to detect the expression of VEGF,MMP-2 and MMP-9 in HCC and normal liver tissues,and the correlation between the expression of VEGF,MMP-2,MMP-9 and clinical pathology and prognosis was analyzed.Results The positive expression rates of VEGF,MMP-2 and MMP-9 in the HCC tissues were significantly higher than those in the normal liver tissues(81.05%,75.79%,84.21%vs.42.22%,33.33%,48.89%)(χ^2=21.364,23.397,19.265,all P<0.05).The positive expression rates of VEGF,MMP-2 and MMP-9 in HCC were significantly increased in patients with poor differentiation,TNM stageⅢ-Ⅳand vascular invasion(all P<0.05).The survival of patients with negative expression of VEGF,MMP-2 and MMP-9 in HCC tissues was significantly better than that of patients with positive expression(logχ^2=3.271,2.975,3.065,all P<0.05).Conclusion The high expression of VEGF,MMP-2 and MMP-9 plays an important role in the growth,invasion and metastasis of HCC tissues.Therefore,VEGF,MMP-2 and MMP-9 can be used as biological indicators of the progression of HCC.
作者
刘海芳
李早红
Liu Haifang;Li Zaohong(Department of Pathology,Yiwu Central Hospital,Yiwu,Zhejiang 322000,China)
出处
《中国基层医药》
CAS
2020年第12期1422-1426,共5页
Chinese Journal of Primary Medicine and Pharmacy
关键词
肝肿瘤
血管内皮生长因子类
基质金属蛋白酶2
基质金属蛋白酶9
临床试验
预后
Liver neoplasms
Vascular endothelial growth factors
Matrix metalloproteinase 2
Matrix metalloproteinase 9
Clinical trial
Prognosis
