摘要
研究了核桃粕乙醇提取物及其萃取物和大孔树脂纯化物的HPLC指纹图谱与降脂作用的谱效关系,为确定核桃粕提取物降脂物质基础提供依据。建立9组核桃粕乙醇提取物及其萃取物和大孔树脂纯化物(S1~S9)HPLC指纹图谱;采用1m M/L游离脂肪酸(Free fatty acid,FFA)诱导Hep G2细胞24 h建立脂肪变性模型,造模成功后考察S1~S9对脂肪变性Hep G2细胞的降脂作用;运用灰色关联度法分析共有峰与降脂的谱效关系和降脂活性成分群。结果表明,S1~S9的HPLC图谱相似度0.31~0.96区间,筛选到15个共有峰,乙酸乙酯萃取物(S5)和大孔树脂纯化物(S8)降脂作用最强(p<0.001),对降脂作用药效贡献大的前6个峰分别为14、6、12、10、13、11,其中14号峰关联度最大且大于0.9,其余5个峰关联度均大于0.85。核桃粕提取物发挥降脂作用是多种成分共同作用的结果。glansreginin A、(4S)-4-羟基-α-四氢萘酮-4-O-β-D-(6′-O-4″-羟基苯甲酰基)吡喃葡萄糖苷、(2E,4E)-8-羟基-2,7-二甲基-2,4-癸二烯-1,10-二酸6’-O-β-d-葡萄糖基吡喃糖基酯、glansregininB、芦丁、槲皮素3-阿拉伯糖苷或槲皮素3-木糖苷对应的物质是核桃粕提取物中的主要降脂活性成分。
The spectrum-activity relationship between HPLC fingerprints and lipid-lowering effects of an ethanol extract, and its liquid-liquid extract and purified product(by macroporous resin), from the defatted walnut meal was studied, to provide a basis for determining the foundation material of their lipid-lowering effects. The HPLC fingerprints(S1~S9) of these extracts and purified products of defatted walnut meal were acquired;A free fatty acid(FFA) was added to HepG2 cells at 1 mM/L and treated for 24 h, to establish a model of steatosis. After the model was successfully established, the lipid-lowering effects of S1~S9 samples on steatosis in Hep G2 cells were examined;The gray correlation method was used to analyze the spectrum-activity relationship between the common spectral peaks and the lipid-lowering effects of the active ingredient group. Results showed that among the 15 common peaks screened from the similarity range of 0.31~0.96 in the HPLC spectra of S1~S9, the ethyl acetate extract(S5) and the purified product of macroporous resin(S8) had the greatest lipid-lowering effects(p<0.001). The first 6 peaks corresponding to the fractions that made a relatively high contribution to the lipid-lowering effect were peak 14, 6, 12, 10, 13, and 11, respectively. The correlation coefficient of peak 14 was the highest(higher than 0.9), with those of the remaining 5 peaks all higher than 0.85. The lipid-lowering effect of the extract from walnut meal resulted from the combined action of multiple constituents. The substances corresponding to glansreginin A,(4 S)-4-hydroxy-α-tetralone-4-O-β-D-(6′-O-4″-hydroxylbenzoyl)-glucopyranoside,(2 E,4 E)-8-hydroxy-2,7-dimethyl-2,4-decadiene-1,10-dioic acid 6’-O-β-d-glucopiranosyl ester, glansreginin B, rutin, and quercetin 3-arabinoside or quercetin 3-xyloside were identified a the main lipid-lowering active components in the extracts from the defatted walnut meal.
作者
王祖坤
马雅鸽
杨婧娟
张希
赵声兰
WANG Zu-kun;MA Ya-ge;YANG Jin-juan;ZHANG Xi;ZHAO Shen-lan(College of traditional Chinese medicine,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China)
出处
《现代食品科技》
EI
CAS
北大核心
2020年第7期105-112,共8页
Modern Food Science and Technology
基金
国家自然科学基金项目(81760735)
云南省科技厅-云南中医药大学联合专项(2017FF116-016,2019FF002-006)
云南省生物科技重大科技专项(2018ZF013)。
关键词
脱脂核桃粕
HEPG2细胞
降脂
谱效关系
提取物
pressed degreased walnut meal
HepG2 cells
lipid-lowering effect
spectrum-activity relationship
extract
