摘要
采用热水回流法对单性木兰叶多糖进行提取,并优化其提取条件,以及研究其对DPPH自由基(DPPH·)清除能力。结果表明,料液比、提取温度、提取时间对单性木兰叶多糖得率均有影响,影响顺序为料液比>提取温度>提取时间;单性木兰叶多糖的最佳提取条件为料液比1:40 g/mL、提取温度90℃、提取时间2.0 h、提取次数3次,在该提取条件下单性木兰叶多糖的得率达6.05%;单性木兰叶多糖对DPPH·具有较好的清除作用,其IC50值为5.85 mg/L。
The extraction of polysaccharides from Magnolia kwangsiensis Figlar&Noot.(M.kwangsiensis)leaf was studied by hot water reflux method,and the extraction condition was optimized.The DPPH·scavenging activity of polysaccharides from M.kwangsiensis leaf have been studied.The results showed that the yield of M.kwangsiensis leaf polyphenols was affected by ratio of material to solvent,extraction temperature and extraction time.The factors of affecting the yield of polysaccharides in order were ratio of material to solvent>extraction temperature>extraction time.The yield was 6.05%under the optimal conditions:ratio of solvent to material of 1:40 g/mL,extraction temperature of 90℃,extraction time of 2.0 hours and extraction times of 3.The extracts of polysaccharides from M.kwangsiensis leaf presented potential scavenging capacity on DPPH·with IC50 value of 5.85 mg/L.
作者
郑燕菲
黄秋萍
罗贤泉
蓝亮美
ZHENG Yanfei;HUANG Qiuping;LUO Xianquan;LAN Liangmei(College of Chemistry and Chemical Engineering,Guangxi Normal University for Nationalities,Chongzuo 532200,China;Department of Electrical and Automotive Engineering,Liuzhou City Vocational College,Liuzhou 545036,China)
出处
《食品科技》
CAS
北大核心
2020年第5期202-206,共5页
Food Science and Technology
基金
广西教育厅2018年广西高校中青年教师基础能力提升项目(2018KY0637)
广西民族师范学院科研项目(2017BS001)
广西教育厅2019年广西高校中青年教师基础能力提升项目(2019KY0769)。
关键词
单性木兰
多糖
提取
DPPH·
Magnolia kwangsiensis Figlar&Noot
polysaccharides
extraction
DPPH·
