摘要
采用两步酶解法制备出海洋胶原肽、海洋骨原肽、海洋蛋白肽、乳清肽、卵白肽、玉米肽、大豆肽7种食源性低聚肽,对其基础理化成分、氨基酸组成和分子量分布进行分析,然后采用火焰原子吸收分光光度法测定低聚肽的持钙能力。成分分析显示:7种低聚肽的蛋白质含量很高,均在80%以上;所有低聚肽均具各自的氨基酸构成特点,不同低聚肽氨基酸含量不同;低聚肽是小分子肽的混合物,以分子量在132~576u之间的肽为主。持钙能力实验结果显示:大豆肽的持钙效果最好,在6mg/mL和10mg/mL的添加浓度下,钙损失率分别为8.55%和5.49%。其它肽在6mg/mL和10mg/mL的添加浓度下,钙损失率均在35.95%至60.20%之间,其中乳清肽持钙效果相对较好。通过评价不同低聚肽的持钙能力,可用于筛选食源性促钙吸收肽,为进一步动物试验或临床试验提供促钙吸收活性依据。
Seven oligopeptides from food protein,including marine collagen peptides,marine osteogenic peptides,marine protein peptides,whey peptides,ovalbumin peptides,corn peptides and soybean peptides were prepared by two-step enzymatic method.Their basic physical and chemical composition,amino acid composition and molecular weight distribution were analyzed,and then their calcium holding capacity was determined by flame atomic absorption spectrophotometry.The composition analysis showed that the protein contents of seven oligopeptides were very high,all of which were over 80%.The amino acid contents of different oligopeptides were different.All of them had a good amino acid composition balance,except corn peptides.Oligopeptides were a mixture of small peptides,most of which were between 132~576 u.The results of calcium holding capacity experiment showed that soybean peptides had the best calcium holding effect,and calcium loss rate was 8.55%and 5.49%at the concentration of 6 mg/mL and 10 mg/m L,respectively.The calcium loss rates of other peptides were between 35.95%and 60.20%at the same concentration,and whey peptides had better calcium retention.After evaluation of calcium holding capacity in different oligopeptides,peptides promoting calcium absorption can be selected and used in future animal or clinical trials.
作者
刘文颖
王憬
潘兴昌
董哲
谷瑞增
蔡木易
LIU Wen-ying;WANG Jing;PAN Xing-chang;DONG Zhe;GU Rui-zeng;CAI Mu-yi(Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province,Ningbo 315832;Beijing Engineering Research Center of Protein and Functional Peptides,China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing 100015)
出处
《中国食品添加剂》
CAS
2020年第7期46-52,共7页
China Food Additives
基金
“十三五”国家重点研发计划(2016YFD0400604)
国家自然科学基金(31671963)
浙江省动物蛋白食品精深加工技术重点实验室开放基金(ZJAP-2019-01)。
关键词
食源性低聚肽
成分分析
持钙能力
促钙吸收作用
food-origin oligopeptides
composition analysis
calcium holding capacity
calcium absorption promoting activity
