摘要
为探讨不同碳源对丹参和藏丹参毛状根的产量和活性成分积累的影响,以丹参和藏丹参的毛状根为试验材料,采用半乳糖、果糖、乳糖、葡萄糖、阿拉伯糖和蔗糖(对照)等6种碳源处理,测定毛状根的鲜重、干重以及丹酚酸类、丹参酮类成分的含量与产量。结果表明,半乳糖对丹参和藏丹参毛状根的生长最为有利,而乳糖和阿拉伯糖不利于2种毛状根生长。对于丹参毛状根,果糖显著促进了丹酚酸B的积累,含量与产量分别比对照组提高了5.801,10.151倍;葡萄糖显著促进了丹酚酸的积累,其中迷迭香酸的含量和产量分别为对照组的7.674,9.260倍,丹酚酸B的含量和产量分别为对照组的5.532,6.675倍。对于藏丹参毛状根,半乳糖显著提高了迷迭香酸的含量和产量,分别达到对照组的7.820,9.944倍;果糖显著促进了隐丹参酮的含量和产量增加,分别达到对照组的9.242,6.609倍。该研究确定了丹参和藏丹参毛状根培养的最佳碳源,为丹参药源性成分的大规模生产以及藏丹参的资源开发利用提供理论支持与指导。
Salvia miltiorrhiza(Sm)and Salvia castanea f.tomentosa(Sc)hairy roots were used as experimental materials to study the effects of six different carbon sources,galactose,fructose,lactose,glucose,arabinose and sucrose(control),on fresh weight,dry weight,contents and yields of salvianolic acids and tanshinones.The results showed that galactose was most beneficial to the growth of two kinds of hairy roots,while lactose and arabinose were not conducive to their growth.As for Sm hairy roots,fructose significantly promoted the accumulation of salvianolic acid B,and the content increased by 5.801 times and 10.151 times compared with the control group,respectively.Glucose significantly promoted the accumulation of salvianolic acids.The content and yield of rosmarinic acid were 7.674 times and 9.260 times of that of the control group,and the content and yield of salvianolic acid B were 5.532 times and 6.675 times of the control group.For the hairy roots of Sc,galactose significantly increased the content and yield of rosmarinic acid,reaching 7.820 times and 9.944 times of the control group,respectively.Fructose promoted the increase of the content and yield of cryptotanshinone,reaching 9.242 times and 6.609 times of the control group,respectively.The study confirmed the optimal carbon source for the hairy root culture of Sm and Sc,and provided theoretical guidance for large-scale production of Sm drug-derived components and the utilization of Sc.
作者
郭妍宏
王飞艳
尤华乾
魏宇坤
杨宗岐
梁宗锁
杨东风
GUO Yan-hong;WANG Fei-yan;YOU Hua-qian;WEI Yu-kun;YANG Zong-qi;LIANG Zong-suo;YANG Dong-feng(Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province,College of Life Science and Medicine,Zhejiang Sci-tech University,Hangzhou 310000,China;Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第11期2509-2514,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81973415,81673536)
上海市资源植物功能基因组学重点实验室开放课题(PFGR201901)。
关键词
丹参
藏丹参
毛状根
碳源
活性成分
Salvia miltiorrhiza
Salvia castanea f.tomentosa
hairy roots
carbon sources
active components
