摘要
对采集自不同地区的21份羊肚菌标本,包括3个梯棱羊肚菌(Morchella importuna)、5个七妹羊肚菌(M.eximia)、10个六妹羊肚菌(M.sextelata)栽培出菇的子囊果,以及3个野生高羊肚菌(M.elata)的子囊果进行多批次组织分离,共计获得348份分离物。对这些分离物的交配型基因检测结果显示:Mat1-1-1和Mat1-2-1两种交配型基因的检出率近似(Mat1-1-1∶Mat1-2-1=267∶278),其中153份(43.96%)分离物只检测到一种交配型基因,每种交配型基因缺失的概率相近,83份缺失交配型基因Mat1-1-1,占23.85%,70份缺失交配型基因Mat1-2-1,占20.11%。七妹羊肚菌19-37和19-43的分离物仅检测到交配型基因Mat1-2-1,而七妹羊肚菌19-44的分离物只检测到交配型基因Mat1-1-1。初步分析组织分离物的培养性状与交配型基因缺失的关联性,发现组织块萌发较慢、气生菌丝稀疏的分离物其交配型基因缺失的概率远高于萌发较快、菌丝浓密的分离物。
Twenty one samples of Morchella spp.,including three cultivated M.importuna samples,five M.eximia samples,ten M.sextelata samples and three wild M.elata samples were subjected to tissue isolation to obtain 348 isolates.These isolates were then analyzed for their mating type gene makers respectively.The results showed that the detection rate of the two representative mating type markers Mat1-1-1 and Mat1-2-1 were similar(Mat1-1-1∶Mat1-2-1=267∶278).There was only one kind of mating genotype detected in 153 isolates,accounting for 43.96%of the total number of the tissue isolates.For these 153 isolates with the other mating genotype missing,the probability of mating gene deletion for Mat1-1-1 and Mat1-2-1 were similar,with 83 isolates missing Mat1-1-1(23.85%)and 70 isolates missing Mat1-2-1(20.11%),respectively.For M.septemtelata 19-37 and M.septemtelata 19-43,only Mat1-2-1 was detected.In contrast,M.septemtelata 19-44 only contained Mat1-1-1.Initial correlation analysis between colony morphology of tissue isolates and the mating type gene deletion phenomenon indicated that isolates with slow germination and colonies of sparse hyphae were more likely to have a deletion of either mating type gene than isolates with fast germination and dense colonies.
作者
刘伟
蔡英丽
何培新
边银丙
LIU Wei;CAI Yingli;HE Peixin;BIAN Yinbing(Institute of Applied Mycology,Huazhong Agricultural University,Wuhan,Hubei 430071,China;Institute of Vegetable,Wuhan Academy of Agricultural Sciences,Wuhan,Hubei 4300345,China;School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou,Henan 450001,China)
出处
《食用菌学报》
CSCD
北大核心
2020年第3期1-6,共6页
Acta Edulis Fungi
基金
湖北省技术创新专项(2019ABB089)。
关键词
羊肚菌
组织分离
菌落萌发
交配型基因
Morel
tissue isolation
colony germination
mating type gene
