摘要
目的:探讨咀嚼对应激大鼠杏仁核、前额叶皮质GABA再摄取的影响。方法:将成年雄性SD大鼠52只分成对照组(CO组)、应激组(ST组)、应激+咀嚼组(SC组)。除CO组外,各组大鼠均置于固定筒中束缚30 min。造模同时,SC组给予直径5 mm木棍以供其咀嚼,咀嚼少于15 min者被丢弃。通过旷场实验记录各组大鼠运动得分以测试其焦虑程度,实验结束后随机挑选大鼠18只(每组6只)立即处死,取杏仁核、前额叶皮质组织检测γ-氨基丁酸转运体-1(GAT-1)mRNA表达情况。结果:与CO组相比,ST组大鼠OF值显著降低,杏仁核与前额叶皮质GAT-1 mRNA表达显著升高(P<0.05);SC组大鼠OF值较ST组显著升高,杏仁核与前额叶皮质GAT-1 mRNA表达显著降低(P<0.05),但与CO组比较差异无统计学意义(P>0.05)。结论:咀嚼可以通过纠正杏仁核、前额叶皮质GAT-1的异常表达调节GABA代谢失常而发挥缓解压力的作用。
Objective:To investigate the effect of mastication on GABA reuptake in amygdala and frontal cortex of rats.Methods:52 SD rats were divided into control group(CO group),stress group(ST group),and stress+chewing group(SC group).Except for CO group,rats in each group were placed in a fixed tube for 30 minutes.Meanwhile,the SC group was given a 5mm diameter stick for chewing,and those chewing for less than 15 minutes were discarded.The motor scores of each group were recorded in Open-Field test.At the end of the experiment,18 rats(6 in each group)were randomly selected and killed immediately.Results:Compared with CO group,the OF value of ST group was significantly lower,and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly higher(P<0.05).The OF value of SC group was significantly higher than ST group,and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly lower(P<0.05).There was no significant difference between SC group and CO group(P>0.05).Conclusion:Mastication can relieve the stress by correcting the abnormal expression of GAT-1 in amygdala and frontal cortex.
作者
杨佳迪
边策
王旭
李荷香
YANG Jiadi;BIAN Ce;WANG Xu;LI Hexiang(Department of Basic Teaching and Research, School of Hospital of Stomatology, Hebei Medical University & Hebei Key laboratory of Stomatology, Shijiazhuang 050017, China;Department of Orthodontics, Beijing Hospital of Stomatology, Capital Medical University, Beijing 100050, China)
出处
《口腔医学研究》
CAS
北大核心
2020年第8期736-740,共5页
Journal of Oral Science Research
关键词
咀嚼
γ-氨基丁酸
Γ-氨基丁酸转运体
应激衰减效应
mastication
γ-aminobutyric acid
γ-aminobutyric acid transporter-1
stress-attenuation effects
