摘要
分别采集成年克隆猪和正常繁殖猪(对照)的肺脏样品,利用双向电泳-质谱体系和Western blot方法的比较蛋白质组学技术对其蛋白进行分离、鉴定和验证,并结合生物信息学手段对相关差异蛋白进行功能分析,研究体细胞核移植(即克隆)操作在蛋白质水平上对克隆猪肺脏发育的影响。结果表明:体细胞核移植操作对克隆猪肺脏的发育有较大影响。与对照猪相比,克隆猪肺脏蛋白共有26个差异蛋白,其中半胱天冬酶凋亡前衔接蛋白、增殖细胞核抗原和核糖-5-磷酸异构酶等22个为上调蛋白,纤维蛋白原γ链、纤维蛋白原β链和血红蛋白α亚基等4个为下调蛋白,主要涉及抗氧化活性、翻译调节和催化活性等重要功能。
Lung samples of adult cloned pigs and normal reproductive pigs(control)were collected,and their proteins were separated,identified and verified by two-dimensional electrophoresis mass spectrometry and Western blot.The function of related differential proteins was analyzed by bioinformatics method to study the effect of somatic cell nuclear transfer(i.e.cloning)on lung development of cloned pigs at protein level.The results showed that somatic cell nuclear transfer had a great influence on the lung development of cloned pigs.Compared with the control pigs,there were 26 differential proteins in the lung of cloned pigs.Among them,22 proteins were up-regulated(such as proapoptotic caspase adaptor protein,proliferating cell nuclear antigen and ribose-5-phosphate isomerasei)and 4 proteins were down-regulated(such as fibrinogenγchain,fibrinogenβchain and hemoglobin subunitα).Those differential proteins were mainly involved in antioxidant activity,translation regulation and catalytic activity.
作者
张树山
戴建军
吴彩凤
孙玲伟
韩雪峻
姜红菊
张德福
ZHANG Shushan;DAI Jianjun;WU Caifeng;SUN Lingwei;HAN Xuejun;JIANG Hongju;ZHANG Defu(Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural Sciences,Division of Animal Genetic Engineering,Shanghai Key Laboratory of Agri-Genetics and Breeding,Shanghai 201106,China;Shanghai Engineering Research Center of Breeding Pig,Shanghai 201302,China)
出处
《上海农业学报》
2020年第4期65-70,共6页
Acta Agriculturae Shanghai
基金
上海市科委“科技创新行动计划”(18391903700)。
关键词
猪
体细胞核移植
肺脏
比较蛋白质组学
Pig
Somatic cell nuclear transfer
Lung
Comparative proteomics
