摘要
比较3种不同栽培技术的鲍姆桑黄孔菌Sanghuangporus baumii——3年生栎树段木鲍姆桑黄孔菌、桑枝代料鲍姆桑黄孔菌和发酵鲍姆桑黄孔菌中多糖、总酚、黄酮及总三萜含量和体外抗肿瘤活性。分别采用硫酸-蒽酮、福林-酚、亚硝酸-硝酸铝和香草醛-冰醋酸法对4种主要成分进行含量测定。选用人大细胞肺癌细胞(H460)、人前列腺癌细胞(PC3)、人乳腺癌细胞(MDA-MB-231)、人肝癌细胞(SMMC-7721和BEL-7402)和人神经母细胞瘤细胞(SH-SY5Y)为待检细胞株,采用CCK-8检测细胞活力。结果显示,发酵鲍姆桑黄孔菌多糖含量最高(29.64%),代料鲍姆桑黄孔菌和3年生段木鲍姆桑黄孔菌多糖含量均低于2.57%;2种代料鲍姆桑黄孔菌的总酚和黄酮含量较高,而发酵鲍姆桑黄孔菌最低(0.24%和0.68%),尤其是代料鲍姆桑黄孔菌1号的总酚和黄酮含量高达4.02%和32.13%;三萜含量在4种栽培法的鲍姆桑黄孔菌中差异不明显。体外对6种肿瘤细胞增殖抑制能力最强的均为代料鲍姆桑黄孔菌,其IC50值最低,其次是3年生段木鲍姆桑黄孔菌,发酵鲍姆桑黄孔菌在本研究设置的浓度范围内无明显的细胞毒性。结果表明:不同栽培方式鲍姆桑黄孔菌产生体外抗肿瘤活性不同,代料鲍姆桑黄孔菌最强,其次是3年生段木鲍姆桑黄孔菌,发酵鲍姆桑黄孔菌活性最差,这种活性差异与其有效成分含量高低直接相关;鲍姆桑黄孔菌发挥抗肿瘤活性的物质基础主要是总酚和黄酮。
Five indicative data(polysaccharide content, polyphenol content, flavonoid content, triterpenoid content, in vitro antitumor activity) of Sanghuangporus baumii grown on oak segment(BO), sawdust of mulberry branch(BS) and artificial media(fermented S. baumii, FB) were evaluated. The spectrophotometric assay via anthrone-sulfuric acid, folin-phend, sodium nitrite-aluminum nitrate, vanillin-glacial acetic acid methods respectively was applied to determine the content of these ingredients. The antiproliferation activities were evaluated by CCK-8 assay on six human cancer cell lines, human large cell lung cancer cell(H460), human prostate cancer cell(PC3), human breast cancer cell(MDA-MB-231), human hepatoma cell(SMMC-7721 and BEL-7402) and human neuroblastoma cell(SH-SY5 Y). The result showed that FB produced the highest content of polysaccharides(29.64%), while the content of polysaccharides from BS and BO were both less than 2.57%. BS-1 and BS-2 were significantly superior in terms of polyphenol content and flavonoid content, especially BS-1 with content of 4.02% and 32.13% respectively. The content of polyphenols and flavonoids from fermented mycelia were the lowest, being 0.24% and 0.68% respectively. The content of triterpenoids had no obvious difference in S. baumii cultivated in different substrates. The most potent antiproliferation ability to the six tumor cells in vitro was BS, followed by BO and FB with no obvious cytotoxicity within the setting concentration range. Different cultivation substrates result in different antitumor activities of S. baumii in vitro, and this difference is directly related to the content of active ingredients. Facts have proved that polyphenols and flavonoids from S. baumii contribute significantly to the anti-tumor capacity.
作者
贺屏雅
杨玥
邸磊
李俊龙
李宁
HE Ping-Ya;YANG Yue;DI Lei;LI Jun-Long;LI Ning(School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第7期1400-1409,共10页
Mycosystema
基金
安徽省科技重大专项课题(16030801112)
安徽医科大学博士科研启动基金(0601087201)。
关键词
鲍姆桑黄孔菌
代料鲍姆桑黄孔菌
抗肿瘤
有效成分
栽培方式
Sanghuangporus baumii
Sanghuangporus baumii grown on sawdust of mulberry branch
anti-tumor
active ingredients
cultivation technique
