摘要
目的:研究毒痰瘀脾虚方含药血清对Hep G2肝癌细胞Wnt/β-catenin信号通路中相关基因及细胞侵袭、迁移、凋亡的影响。方法:采用RT-qPCR实验观察毒痰瘀脾虚方含药血清对Hep G2肝癌细胞Wnt/β-catenin信号通路中β-catenin mRNA、MMP2 mRNA、Cyclin D1 mRNA表达的影响;Transwell细胞侵袭和迁移实验,观察其对Hep G2肝癌细胞侵袭和迁移的影响;AnnexinV-FITC双染流式细胞法检测其对Hep G2肝癌细胞凋亡的影响。结果:RT-qPCR结果显示,与阴性组比较,毒痰瘀脾虚方含药血清能下调HepG2肝癌细胞Wnt/β-catenin信号通路中β-catenin mRNA、MMP2 mRNA、Cyclin D1 mRNA的表达,差异有统计学意义(P<0.01或P<0.05);Transwell细胞侵袭实验和迁移实验结果显示,毒痰瘀脾虚方含药血清能抑制Hep G2细胞的侵袭(P<0.01),对细胞的迁移无明显影响(P>0.05);细胞凋亡实验结果显示,毒痰瘀脾虚方含药血清能促进Hep G2细胞的凋亡,与阴性组比较,差异有统计学意义(P<0.01)。结论:毒痰瘀脾虚方含药血清能抑制Hep G2细胞侵袭,促进细胞凋亡,并能抑制Wnt/β-catenin信号通路中β-catenin mRNA、MMP2 mRNA、Cyclin D1 mRNA表达。
Objective:To study the effects of drug-containing serum of Dutanyu Pixu formulae on invasion,migration and apoptosis of Hep G2 liver cancer cells,and related genes in Wnt/β-catenin signaling pathway.Methods:RT-qPCR experiment was used to observe the effect of drug-containing serum of Dutanyu Pixu formulae onβ-catenin mRNA,MMP2 mRNA and Cyclin D1 mRNA expression in Wnt/β-catenin signaling pathway of Hep G2 hepatoma cells.The invasion and migration experiments of Transwell cells were carried out to observe their effects on the invasion and migration of Hep G2 hepatoma cells.AnnexinV-FITC double staining flow cytometry was used to detect its effect on apoptosis of Hep G2 hepatoma cells.Results:RT-qPCR results showed that,compared with the negative group,the drug-containing serum of Dutanyu Pixu formulae could down-regulate the mRNA expression ofβ-catenin,MMP2 and cyclinD1 in the Wnt/β-catenin signaling pathway of Hep G2 hepatoma cells,and the difference was statistically significant(P<0.05).The results of Transwell cell invasion and migration experiments showed that the drug-containing serum of Dutanyu Pixu formulae could inhibit the invasion of Hep G2 hepatoma cells(P<0.01),and had no significant effect on the migration of liver cancer cells(P>0.05).The results of apoptosis experiments showed that the drug-containing serum of Dutanyu Pixu formulae could promote the apoptosis of Hep G2 cells.Compared with the negative group,the difference was statistically significant(P<0.01).Conclusion:The drug-containing serum of Dutanyu Pixu formulae can inhibit the invasion,migration of Hep G2 hepatoma cells,and promote the apoptosis of hepatoma cells,and inhibit the expression ofβ-catenin mRNA,MMP2 mRNA and cyclinD1 mRNA in the Wnt/β-catenin signaling pathway.
作者
潘谊
吴辉坤
皇甫炎林
黄海唐
PAN Yi;WU Hui-kun;HUANGFU Yan-lin;HUANG Hai-tang(Hubei University of Traditional Chinese Medicine,Wuhan Hubei 430065,China;Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan Hubei 430061,China)
出处
《中医药导报》
2020年第11期38-41,45,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家中医药管理局资助项目(JDZX2015177),项目名称:基于毒痰瘀虚证候要素的H22肝癌荷瘤小鼠方证相关研究。
