摘要
建立一种快速、灵敏的检测葡萄酒中醋酸菌群的检测技术。通过改进葡萄酒中脱氧核糖核酸(DNA)提取方法,设计醋酸菌通用引物,优化实时荧光定量聚合酶链式反应(RT-FQPCR)条件,确定葡萄酒中醋酸菌的快速检测方法,同时利用7种醋酸菌对该方法的通用性、特异性以及灵敏度进行了验证。结果表明,7种醋酸菌均具有明显扩增曲线;非目的菌在该种条件下未见扩增,综合检测灵敏度为124 CFU/mL。以10种市售葡萄酒为基质,在其中添加醋酸菌均可检测出目标添加菌株。该方法通用性强,可用于葡萄酒中醋酸菌群的检测。
A rapid and sensitive detection technique for acetic acid bacteria flora in wine was established by improving the extraction method of DNA in wine.The universal primers for acetic acid bacteria were designed,and the reaction conditions were optimized of real-time fluorescence quantitative polymerase chain reaction(RT-FQPCR).At the same time,the universality,specificity and sensitivity of the method were verified using 7 acetic acid bacteria strains.The results showed that 7 strains had obvious amplification curves,non-target bacteria were not amplified under this condition,and the comprehensive detection sensitivity was 124 CFU/ml.Based on 10 commercial wines,the target strains could be detected by adding acetic acid bacteria,indicating the method had high universality and could be used for detecting acetic acid bacteria flora in wine.
作者
李斌
钱云开
肖艳霞
单超
柳吉芹
高飞
王海洋
王传现
邹静
阎贺静
LI Bin;QIAN Yunkai;XIAO Yanxia;SHAN Chao;LIU Jiqin;GAO Fei;WANG Haiyang;WANG Chuanxian;ZOU Jing;YAN Hejing(Technical Center of Qinhuangdao Customs,Qinhuangdao 066004,China;College of Food Science,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;Technology Center of Shanghai Customs Animal and Plant and Food Inspection and Quarantine,Shanghai 200135,China)
出处
《中国酿造》
CAS
北大核心
2020年第8期198-201,共4页
China Brewing
基金
国家质量监督检验检疫总局科技计划项目(2016IK105,2018IK005,2015IK226)
河北省重点研发计划项目(19227119D,19222810D)
河北省自然基金项目(C2017407041)
保税区管理局自贸区专项发展资金项目(中(沪)自保管[2016]192号)
上海市科委技术标准专项项目(14DZ0501001)。
关键词
醋酸菌
实时荧光定量聚合酶链式反应
葡萄酒
检测
acetic acid bacteria
real-time fluorescence quantitative polymerase chain reaction
wine
detection
